miR-4319 通过靶向 ABTB1 抑制结直肠癌细胞的进展。
MiR-4319 suppresses colorectal cancer progression by targeting ABTB1.
机构信息
Nanjing Medical University, Nanjing, China.
Department of General Surgery, Wuxi People's Hospital, Wuxi, China.
出版信息
United European Gastroenterol J. 2019 May;7(4):517-528. doi: 10.1177/2050640619837440. Epub 2019 Mar 6.
BACKGROUND
Colorectal cancer is one of the highly malignant cancers with a poor prognosis. The exact mechanism of colorectal cancer progression is not completely known. Recently, microRNAs (miRNAs, miRs) were suggested to participate in the regulation of multiple cancer development, including colorectal cancer.
METHODS
MiR-4319 expression in colorectal cancer patient samples was detected by real-time polymerase chain reaction. MiR-4319 was knocked down in the colorectal cancer cells by siRNA transfection to study the role of miR-4319 in the cell cycle and proliferation of colorectal cancer cells.
RESULTS
MiR-4319 expression was found to be inverse correlated with survival in colorectal cancer patients. Overexpression of miR-4319 markedly reduced the proliferation of colorectal cancer cells and altered cell cycle distribution. A further experiment showed that ABTB1 is the target gene of miR-4319. MiR-4319 was regulated by PLZF.
CONCLUSION
Our studies indicated that reduced expression of miR-4319 was correlated with poor prognosis in colorectal cancer patients; miR-4319 also suppressed colorectal cancer cell proliferation by targeting ABTB1. ABTB1 might become an excellent therapeutic target for colorectal cancer treatment.
背景
结直肠癌是预后较差的高度恶性癌症之一。结直肠癌进展的确切机制尚不完全清楚。最近，microRNAs（miRNAs，miRs）被认为参与了多种癌症的发展调控，包括结直肠癌。
方法
通过实时聚合酶链反应检测结直肠癌患者样本中的 miR-4319 表达。通过 siRNA 转染使结直肠癌细胞中的 miR-4319 敲低，研究 miR-4319 在结直肠癌细胞周期和增殖中的作用。
结果
miR-4319 的表达与结直肠癌患者的生存呈负相关。miR-4319 的过表达显著降低了结直肠癌细胞的增殖并改变了细胞周期分布。进一步的实验表明，ABTB1 是 miR-4319 的靶基因。PLZF 调节 miR-4319。
结论
我们的研究表明，miR-4319 的表达降低与结直肠癌患者的预后不良相关；miR-4319 还通过靶向 ABTB1 抑制结直肠癌细胞的增殖。ABTB1 可能成为结直肠癌治疗的优秀治疗靶点。
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