两种抗菌肽与两种常用抗生素协同作用的初步研究
A Pilot Study of the Synergy between Two Antimicrobial Peptides and Two Common Antibiotics.
作者信息
Kampshoff Franziska, Willcox Mark D P, Dutta Debarun
机构信息
School of Life Science, Engineering & Design, Saxion University of Applied Science, 7513 AB Ensched Enschede, The Netherlands.
School of Optometry and Vision Science, University of New South Wales, Sydney, NSW 2052, Australia.
出版信息
Antibiotics (Basel). 2019 May 9;8(2):60. doi: 10.3390/antibiotics8020060.
BACKGROUND
Frequent and unrestricted use of antibiotics has been associated with the development of antibiotic resistance by microorganisms. Thus, there is a need to find novel antibacterial agents or a combination of agents as the first line of treatment for various infections. This study aimed to investigate the synergy between antimicrobial peptide (AMP) combinations or between AMP-antibiotics combinations using two common pathogens, and .
METHODS
The AMPs melimine, Mel4 and protamine, and antibiotics cefepime and ciprofloxacin were used in this study. The minimum inhibitory concentration (MIC) of each were evaluated against and strains by a microtiter broth dilution. Based on the MIC of each antimicrobial agent, a checkerboard assay was performed to investigate the synergy between them, which was expressed as the fractional inhibitory concentration (FIC).
RESULTS
The combination of melimine and ciprofloxacin showed synergistic activity against antibiotic sensitive or resistant strains of and with FIC values ≤0.5.
CONCLUSION
Combinations of AMPs and the fluoroquinolone ciprofloxacin is a promising method for reducing resistance to the fluoroquinolone of .
背景
抗生素的频繁和无节制使用与微生物抗生素耐药性的产生有关。因此，有必要寻找新型抗菌剂或联合用药作为各种感染的一线治疗方法。本研究旨在利用两种常见病原体研究抗菌肽（AMP）组合之间或AMP与抗生素组合之间的协同作用。
方法
本研究使用了抗菌肽三聚氰胺、Mel4和鱼精蛋白，以及抗生素头孢吡肟和环丙沙星。通过微量肉汤稀释法评估每种药物对[具体病原体1]和[具体病原体2]菌株的最低抑菌浓度（MIC）。根据每种抗菌剂的MIC，进行棋盘法试验以研究它们之间的协同作用，用分数抑菌浓度（FIC）表示。
结果
三聚氰胺和环丙沙星的组合对[具体病原体1]和[具体病原体2]的抗生素敏感或耐药菌株显示出协同活性，FIC值≤0.5。
结论
抗菌肽与氟喹诺酮类环丙沙星联合使用是降低[具体病原体]对氟喹诺酮耐药性的一种有前景的方法。
Zotero 插件