质谱流式细胞术数据的采集、处理与质量控制

Acquisition, Processing, and Quality Control of Mass Cytometry Data.

作者信息

Lee Brian H, Rahman Adeeb H

机构信息

Human Immune Monitoring Center, Icahn School of Medicine at Mt. Sinai, New York, NY, USA.

Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mt. Sinai, New York, NY, USA.

出版信息

Methods Mol Biol. 2019;1989:13-31. doi: 10.1007/978-1-4939-9454-0_2.

DOI:10.1007/978-1-4939-9454-0_2

PMID:31077096

Abstract

Mass cytometry uniquely combines the principles of mass spectrometry and flow cytometry for high dimensional profiling of immune cells at a single cell level. Using isotopically conjugated antibodies, mass cytometry overcomes the limitations of spectral overlap associated with flow cytometry and allows for deeper single cell characterization of complex biospecimens using more cellular markers. However, the nature of mass spectrometry-based single cell measurements requires specific considerations in acquiring and processing data. This chapter provides an overview of how to optimally acquire mass cytometry data and how to process this data for subsequent analysis and characterization of cell populations.

摘要

质谱流式细胞术独特地结合了质谱分析和流式细胞术的原理，可在单细胞水平上对免疫细胞进行高维分析。通过使用同位素偶联抗体，质谱流式细胞术克服了与流式细胞术相关的光谱重叠限制，并允许使用更多细胞标志物对复杂生物样本进行更深入的单细胞表征。然而，基于质谱的单细胞测量的性质在获取和处理数据时需要特殊考虑。本章概述了如何最佳地获取质谱流式细胞术数据以及如何处理这些数据以用于后续细胞群体的分析和表征。