HIV 的 RNA 包装。
RNA Packaging in HIV.
机构信息
HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702-1201, USA.
出版信息
Trends Microbiol. 2019 Aug;27(8):715-723. doi: 10.1016/j.tim.2019.04.003. Epub 2019 May 10.
Successful replication of the AIDS retrovirus, HIV, requires that its genomic RNA be packaged in assembling virus particles with high fidelity. However, cellular mRNAs can also be packaged under some conditions. Viral RNA (vRNA) contains a 'packaging signal' (ψ) and is packaged as a dimer, with two vRNA monomers joined by a limited number of base pairs. It has two conformers, only one of which is capable of dimerization and packaging. Recent years have seen important progress on the 3D structure of dimeric ψ. Gag, the protein that assembles into the virus particle, interacts specifically with ψ, but this is obscured under physiological conditions by its high nonspecific affinity for any RNA. New results suggest that vRNA is selected for packaging because ψ nucleates assembly more efficiently than other RNAs.
成功复制艾滋病逆转录病毒（HIV）需要其基因组 RNA 以高保真度包装在组装的病毒颗粒中。然而，在某些条件下，细胞 mRNA 也可以被包装。病毒 RNA（vRNA）包含一个“包装信号”（ψ），并作为二聚体被包装，两个 vRNA 单体通过有限数量的碱基对连接。它有两种构象，只有一种能够二聚化和包装。近年来，关于二聚体 ψ 的 3D 结构取得了重要进展。Gag 蛋白组装成病毒颗粒，与 ψ 特异性相互作用，但在生理条件下，由于其对任何 RNA 的高非特异性亲和力而被掩盖。新的结果表明，vRNA 被选择进行包装是因为 ψ 比其他 RNA 更有效地引发组装。
Zotero 插件