姜黄素在抗肿瘤光动力治疗中的作用：Caco-2 和 PC-3 癌细胞系的体外实验。

The effect of curcumin in antitumor photodynamic therapy: In vitro experiments with Caco-2 and PC-3 cancer lines.

机构信息

Boğaziçi University, Institute of Biomedical Engineering, Istanbul, Turkey.

出版信息

Photodiagnosis Photodyn Ther. 2019 Sep;27:95-99. doi: 10.1016/j.pdpdt.2019.05.012. Epub 2019 May 14.

DOI:10.1016/j.pdpdt.2019.05.012

PMID:31100447

Abstract

BACKGROUND

Photodynamic Therapy (PDT) is a promising antitumor and anti-bacterial treatment method for its high selectivity, non-invasiveness, and minimal side effects. However, cellular mechanisms may lead to PDT resistance and thus effect efficacy. The aim of this study is to test whether Curcumin, which is a non-toxic natural compound that has antitumor characteristics, can increase PDT efficacy by overcoming the resistance of cancer cells.

METHODS

5-ALA mediated PDT was tested on two cell lines, PC-3 and Caco-2. Curcumin toxicity was evaluated at different concentrations. The determined PDT doses were applied to the cell lines together with two different Curcumin concentrations. Cell viability was evaluated by MTT assay, 24 hs after the treatments. Results were evaluated using One-Way ANOVA followed by post-hoc tests.

RESULTS

Using non-toxic doses of Curcumin resulted in a significant decrease in PDT resistance in Caco-2 cells and thus increased the efficacy of 5-ALA mediated PDT, but not on PC-3. Adding Curcumin to 5-ALA mediated PDT led to more effective results on Caco-2 with a 62.4% decrease in cell viability. On the other hand, adding Curcumin to 5-ALA mediated PDT on PC-3 cells didn't produce statistically significant increase in efficacy with a 36% decrease in cell viability.

CONCLUSION

5-ALA mediated PDT combined with Curcumin synergistically enhanced antitumor PDT efficacy on Caco-2, which is considered a highly resistive cancer cell line.

摘要

背景

光动力疗法（PDT）是一种很有前途的抗肿瘤和抗细菌治疗方法，因为它具有高选择性、非侵入性和最小的副作用。然而，细胞机制可能导致 PDT 耐药性，从而影响疗效。本研究旨在测试姜黄素（一种具有抗肿瘤特性的无毒天然化合物）是否可以通过克服癌细胞的耐药性来提高 PDT 的疗效。

方法

在两种细胞系 PC-3 和 Caco-2 上测试了 5-ALA 介导的 PDT。在不同浓度下评估了姜黄素的毒性。将确定的 PDT 剂量与两种不同浓度的姜黄素一起应用于细胞系。用 MTT 测定法评估细胞活力，在治疗后 24 小时。使用 One-Way ANOVA 进行评估，然后进行事后检验。

结果

使用非毒性剂量的姜黄素可显著降低 Caco-2 细胞中的 PDT 耐药性，从而提高 5-ALA 介导的 PDT 的疗效，但对 PC-3 细胞无效。将姜黄素添加到 5-ALA 介导的 PDT 中，可使 Caco-2 的疗效更有效，细胞活力降低 62.4%。另一方面，将姜黄素添加到 5-ALA 介导的 PDT 中对 PC-3 细胞的疗效没有产生统计学上的显著增加，细胞活力降低 36%。