人细胞毒性自然杀伤细胞：一种新型非病毒方法，可实现高效转染和强烈杀伤肿瘤细胞。
Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing.
机构信息
Immunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, Italy.
Department of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, Rome, Italy.
出版信息
Front Immunol. 2019 Apr 30;10:957. doi: 10.3389/fimmu.2019.00957. eCollection 2019.
CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.
CAR-NK 细胞可能是过继免疫疗法治疗白血病和实体瘤的一种有价值的工具，可作为 CAR-T 细胞的补充。然而，将基因转入人自然杀伤（NK）细胞是一项具有挑战性的任务，特别是在非病毒技术方面。在这里，我们描述了一种新的程序，可在静止或细胞因子扩增的人 NK 细胞群体和 NK-92 细胞系中，通过电穿孔有效地转染质粒 DNA，包括 CAR 和 CCR7 基因。该程序可为 CAR-NK 细胞在癌症的过继免疫疗法中的安全有效应用提供合适的平台。
Zotero 插件