Pharmacological and Anti-inflammatory Evaluation of Xerophyte Crantz.

Known for centuries throughout the world, species have long been used as traditional herbal remedies for many diseases related to inflammatory conditions of the skin, respiratory and digestive tract, or even malignancy. This study is aimed first at investigating the antioxidant and regenerative effects of Crantz hydroalcoholic extract followed by an experiment using a turpentine oil-induced inflammation model. The evaluation for antioxidant activity was performed using classical assays such as DPPH and TEAC scavenging assays but also EPR, and the total phenolic content was determined using the Folin-Ciocalteu reagent. The wound healing assay was performed on human cells (Human EA.hy926). Besides, the prooxidant activity was determined using a method which involves free radical generation by laccase and the oxidation of haemoglobin. On turpentine oil-induced inflammation in rats, the effects of three doses of extracts (100%, 50%, and 25%) were assessed by measuring oxidative stress (MDA, TOS, OSI, NO, CAT, and SOD) and inflammatory (CRP, WBC, and NEU) parameters. Having a rich polyphenolic content, the xerophyte exhibited a strong antioxidant activity by scavenging radicals, enhancing cell regeneration, and reducing oxidative stress markers. Diluted extract (25%) exhibited the best antioxidant, wound healing, and anti-inflammatory activity.