泰国北部健康人和猪中分离出的产超广谱β-内酰胺酶菌株的多位点序列分型及特征分析
Multilocus sequence typing and characterization of extended-spectrum beta-lactamase-producing isolated from healthy humans and swine in Northern Thailand.
作者信息
Seenama Chakkraphong, Thamlikitkul Visanu, Ratthawongjirakul Panan
机构信息
Program of Molecular Sciences in Medical Microbiology and Immunology, Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
出版信息
Infect Drug Resist. 2019 Jul 19;12:2201-2214. doi: 10.2147/IDR.S209545. eCollection 2019.
PURPOSE
Here, we investigated the genetic relationships and characteristics of extended- spectrum beta-lactamase-producing (ESBL-) isolates from healthy hosts, humans in the community and swine among the livestock of Amphor Mueang, Lamphun Province, Thailand.
PATIENTS AND METHODS
Four hundred and nine rectal swabs were collected from healthy people and swine. A total of 212 ESBL- was isolated and phenotypically confirmed by a combination disk method. Putative ESBL-encoding genes, including , , and , were examined by multiplex-PCR. Randomly selected 42 ESBL- isolates were whole genome sequenced to characterize the ESBL-encoding genes and identify additional antimicrobial resistance genes. The genetic relatedness of 212 ESBL- was investigated by multilocus sequence typing.
RESULTS
Overall, was the dominant ESBL-encoding gene found in 95.75% of the isolates, followed by (60.85%) and (2.40%). While was the most common subgroup found in this study. Whole genome sequencing showed a total of 15 different antimicrobial resistance genes other than , including , among the selected 42 ESBL- isolates. Over half of the ESBL- (56.60%) carried co-existing with . The most common sequence types (STs) identified from human isolates were ST131, ST101, and ST70 while those isolated from swine were ST10, ST48, and ST131. ST131 strains carrying were the major isolated ESBL- strains, supporting a previous study that considered this strain truly pathogenic. Noticeably, 66.51% of ESBL- strains shared 19 identical STs, including a host-restricted ST131 between humans and swine, suggesting that transmission between these two hosts might be possible.
CONCLUSION
Proof of a direct transfer of ESBL- from animals to humans, or vice versa, is required for further elucidation. The ESBL- isolated from both types of healthy hosts may serve as a reservoir for community-acquired antimicrobial resistance.
目的
在此，我们调查了泰国南奔府安帕莫昂地区健康宿主（社区人群）和猪（家畜）中产超广谱β-内酰胺酶（ESBL）菌株的遗传关系及特征。
患者与方法
从健康人和猪中采集了409份直肠拭子。共分离出212株产ESBL菌株，并通过复合纸片法进行表型确认。采用多重聚合酶链反应检测包括blaCTX-M、blaTEM和blaSHV在内的假定ESBL编码基因。随机选择42株产ESBL菌株进行全基因组测序，以鉴定ESBL编码基因并确定其他抗菌药物耐药基因。通过多位点序列分型研究212株产ESBL菌株的遗传相关性。
结果
总体而言，blaCTX-M是在95.75%的分离株中发现的主要ESBL编码基因，其次是blaTEM（60.85%）和blaSHV（2.40%）。而blaCTX-M-15是本研究中最常见的blaCTX-M亚组。全基因组测序显示，在所选的42株产ESBL菌株中，除blaCTX-M外，共有15种不同的抗菌药物耐药基因，包括blaTEM。超过一半的产ESBL菌株（56.60%）携带blaTEM并与blaCTX-M共存。从人类分离株中鉴定出的最常见序列类型（STs）为ST131、ST101和ST70，而从猪中分离出的为ST10、ST48和ST131。携带blaCTX-M-15的ST131菌株是主要分离的产ESBL菌株，支持了先前一项认为该菌株具有真正致病性的研究。值得注意的是，66.51%的产ESBL菌株共有19种相同的STs，包括人和猪之间宿主限制性的ST131，这表明这两种宿主之间可能存在传播。
结论
需要进一步阐明ESBL从动物直接传播给人类或反之亦然的证据。从这两种健康宿主中分离出的产ESBL菌株可能是社区获得性抗菌药物耐药性的储存库。
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