一种基于丹磺酰肽的新型汞（II）离子荧光化学传感器及其在实际水样和活的LNcap细胞中的应用。
A novel fluorescent chemosensor for detection of mercury(II) ions based on dansyl-peptide and its application in real water samples and living LNcap cells.
作者信息
Xue Shirui, Wang Peng, Chen Kai
机构信息
School of Journalism and Communications, China West Normal University, Shida Road 1#, Nanchong, 637009, PR China.
Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong, 637009, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Feb 5;226:117616. doi: 10.1016/j.saa.2019.117616. Epub 2019 Oct 7.
Mercury is one of the most hazardous pollutants, and mercury pollution is a serious hazard to our environment. Herein, we designed and synthesized a new peptide-based fluorescent chemosensor (L) based on a Fmoc-Lys (Fmoc)-OH backbone conjugated with two Serines and dansyl groups using solid phase peptide synthesis (SPPS) technology. L exhibited highly selective and excellent sensitive detection of Hg ions in 100% aqueous solutions through fluorescence quenching. The chemosensor L forms a 2:1 stoichiometry with high binding constants (4.89×10M) and the detection limit for Hg ions of the proposed assay was 7.59nM. In addition, the recovery test results of Hg concentration in actual water samples showed that the quantitative detection of Hg ions can be realized in two water samples. Moreover, L showed low cytotoxicity and excellent membrane permeability in HK2 cells, which has been successfully applied for monitoring Hg ions in living LNCaP cells.
汞是最具危害性的污染物之一，汞污染对我们的环境构成严重危害。在此，我们利用固相肽合成（SPPS）技术，设计并合成了一种基于Fmoc-Lys（Fmoc）-OH主链、与两个丝氨酸和丹磺酰基共轭的新型肽基荧光化学传感器（L）。L通过荧光猝灭在100%水溶液中对Hg离子表现出高度选择性和优异的灵敏检测。化学传感器L与Hg离子形成2:1的化学计量比，具有高结合常数（4.89×10M），该检测方法对Hg离子的检测限为7.59nM。此外，实际水样中Hg浓度的回收率测试结果表明，在两个水样中均可实现对Hg离子的定量检测。而且，L在HK2细胞中表现出低细胞毒性和优异的膜通透性，已成功应用于监测活LNCaP细胞中的Hg离子。
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