比率型和比色型半花菁荧光探针用于检测 SO 衍生物及其在生物成像中的应用。
A Ratiometric and Colorimetric Hemicyanine Fluorescent Probe for Detection of SO Derivatives and Its Applications in Bioimaging.
机构信息
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Experiment Center of Teaching & Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
出版信息
Molecules. 2019 Nov 5;24(21):4011. doi: 10.3390/molecules24214011.
Based upon the intramolecular charge transfer (ICT) mechanism, a novel ratiometric fluorescent probe was developed to detect SO/HSO. The probe displayed both colorimetric and ratiometric responses toward SO/HSO. It displayed a quick response (within 60 s), good selectivity and high sensitivity (a detection limit of 28 nM) towards SO/HSO. The SO/HSO sensing mechanism was confirmed as the Michael addition reaction by ESI-MS. Moreover, the probe could be applied to measure the level of sulfite in real samples, like sugar and chrysanthemum, and it could also be used to detect SO/HSO in HepG2 cells through confocal fluorescence microscopy, which proved its practical application in clinical diagnosis.
基于分子内电荷转移（ICT）机制，设计了一种新型的比率荧光探针用于检测 SO/HSO。该探针对 SO/HSO 具有比色和比率响应。它对 SO/HSO 具有快速响应（在 60 秒内）、良好的选择性和高灵敏度（检测限为 28 nM）。通过 ESI-MS 证实了 SO/HSO 的传感机制为迈克尔加成反应。此外，该探针可用于测定实际样品（如糖和菊花）中的亚硫酸盐含量，也可通过共聚焦荧光显微镜用于检测 HepG2 细胞中的 SO/HSO，这证明了其在临床诊断中的实际应用。
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