利用 SBA-15 负载的Thermomyces lanuginosus 脂肪酶进行甘油脂解生产二酰基甘油。

Production of diacylglycerols through glycerolysis with SBA-15 supported Thermomyces lanuginosus lipase as catalyst.

机构信息

Research Center for Environmental Ecology and Engineering, Key Laboratory for Green Chemical Process of Ministry of Education, Key Laboratory for Hubei Novel Reactor & Green Chemical Technology, School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan, China.

School of Food Science, Guangdong Pharmaceutical University, Zhongshan, China.

出版信息

J Sci Food Agric. 2020 Mar 15;100(4):1426-1435. doi: 10.1002/jsfa.10140. Epub 2019 Dec 20.

DOI:10.1002/jsfa.10140

PMID:31710696

Abstract

BACKGROUND

In this study, SBA-15 was functionalized by silane coupling reagents, then lipase from Thermomyces lanuginosus (TLL) was immobilized onto the parent and the organically modified SBA-15 for diacylglycerol (DAG) production through glycerolysis.

RESULTS

Diacylglycerol content of 54.77 ± 0.63%, and triacylglycerol (TAG) conversion of 77.75 ± 1.24%, were obtained from the parent SBA-15 supported TLL-mediated glycerolysis reaction in a solvent-free system. However, poor performance was unexpectedly observed when co-solvents were introduced into the reaction system. After organic modification, the functionalized SBA-15 supported TLL samples all exhibited reasonable performance, producing DAG content over 40 wt% and TAG conversion over 70 wt%. Higher DAG content, up to 59.19 ± 1.10%, was observed from the phenyl group-modified SBA-15 supported TLL. The operational stability of the immobilized TLL samples in glycerolysis was also improved after organic functionalization. The phenyl group-modified SBA-15 supported TLL showed good reusability in the present glycerolysis reaction, and 95.21 ± 4.87% of the initial glycerolysis activity remained after five cycles of reuse.

CONCLUSION

The organic modification of SBA-15 improved the catalytic performance of its supported TLL in glycerolysis, in terms of TAG conversion, DAG content, and reusability. © 2019 Society of Chemical Industry.

摘要

背景

在这项研究中，通过硅烷偶联试剂对 SBA-15 进行功能化，然后将脂肪酶从Thermomyces lanuginosus（TLL）固定在母体和有机修饰的 SBA-15 上，通过甘油解生产二酰基甘油（DAG）。

结果

在无溶剂体系中，通过母体 SBA-15 负载 TLL 介导的甘油解反应，可获得 54.77±0.63%的二酰基甘油含量和 77.75±1.24%的三酰基甘油（TAG）转化率。然而，当引入共溶剂进入反应体系时，却观察到较差的性能。经过有机修饰后，功能化的 SBA-15 负载 TLL 样品均表现出合理的性能，生成的 DAG 含量超过 40wt%，TAG 转化率超过 70wt%。在苯基改性的 SBA-15 负载 TLL 中，观察到更高的 DAG 含量，达到 59.19±1.10%。在甘油解中，固定化 TLL 样品的操作稳定性也经过有机功能化得到提高。苯基改性的 SBA-15 负载 TLL 在本甘油解反应中表现出良好的可重复使用性，重复使用五次后，初始甘油解活性仍保留 95.21±4.87%。