CgRel 通过调节太平洋牡蛎 Crassostrea gigas 中 CgIL17s 和 CgBigDef1 的产生来参与抗菌免疫。
CgRel involved in antibacterial immunity by regulating the production of CgIL17s and CgBigDef1 in the Pacific oyster Crassostrea gigas.
机构信息
Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China.
Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.
出版信息
Fish Shellfish Immunol. 2020 Feb;97:474-482. doi: 10.1016/j.fsi.2019.11.036. Epub 2019 Nov 16.
The NF-κB/Rel transcription factors play essential roles in the induction and regulation of innate immune responses. In the present study, the full-length cDNA of CgRel from the Pacific oyster Crassostrea gigas was of 2,647 bp with an RHD and an IPT domain. The mRNA of CgRel was found to be constitutively expressed in all the tested tissues including gills, hepatopancreas, gonad, adductor muscle, labial palps, mantle, hemocytes, and ganglion. After lipopolysaccharide (LPS) stimulation, the expression level of CgRel mRNA in hemocytes was up-regulated to the first peak at 3 h (3.06-fold compared to the control group, p < 0.001) and second peak at 48 h (1.96-fold, p < 0.05). It increased significantly at 3 h (7.68-fold compared to the control group, p < 0.001), 24 h (3.63-fold, p < 0.05) and 48 h (1.99-fold, p < 0.05) post Vibrio splendidus stimulation, respectively. The protein of CgRel was translocated from cytoplasm into nucleus of oyster hemocytes after LPS stimulation. The mRNA expression levels of interleukin17s (CgIL17s) and big defensin (CgBigDef1) in hemocytes were examined after the expression of CgRel was silenced by RNAi. The transcripts of CgIL17-1 (0.25-fold of the control group, p < 0.01), CgIL17-2 (0.12-fold, p < 0.01), CgIL17-4 (0.33-fold, p < 0.01), CgIL17-6 (0.27-fold, p < 0.05) and CgBigDef1 (0.38-fold, p < 0.01) in CgRel-knockdown oysters decreased significantly at 12 h after LPS stimulation. The results indicated that CgRel played important roles in the immune defense against bacteria by regulating the expression of CgIL17 and CgBigDef1.
NF-κB/Rel 转录因子在先天免疫反应的诱导和调节中发挥重要作用。本研究从太平洋牡蛎 Crassostrea gigas 中克隆得到全长 2647bp 的 CgRel cDNA，其包含 RHD 和 IPT 结构域。CgRel mRNA 在检测的所有组织中均有组成性表达，包括鳃、肝胰腺、性腺、闭壳肌、唇瓣、套膜、血细胞和神经节。脂多糖（LPS）刺激后，血细胞中 CgRel mRNA 的表达水平在 3 小时（与对照组相比，3.06 倍，p<0.001）和 48 小时（1.96 倍，p<0.05）达到第一个峰值。在 V. splendidus 刺激后 3 小时（与对照组相比，7.68 倍，p<0.001）、24 小时（3.63 倍，p<0.05）和 48 小时（1.99 倍，p<0.05）显著增加。LPS 刺激后，CgRel 蛋白从牡蛎血细胞的细胞质转位到细胞核。沉默 CgRel 表达后，检测血细胞中白细胞介素 17s（CgIL17s）和大防御素（CgBigDef1）的 mRNA 表达水平。在 LPS 刺激后，CgRel 敲低的牡蛎中 CgIL17-1（对照组的 0.25 倍，p<0.01）、CgIL17-2（0.12 倍，p<0.01）、CgIL17-4（0.33 倍，p<0.01）、CgIL17-6（0.27 倍，p<0.05）和 CgBigDef1（0.38 倍，p<0.01）的转录本在 12 小时后显著降低。结果表明，CgRel 通过调节 CgIL17 和 CgBigDef1 的表达在细菌免疫防御中发挥重要作用。
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