取卵前两天制备体外受精培养基会影响胚胎学参数吗？一项回顾性病例对照研究。
Can We Prepare IVF Culture Media Two Days Before Ovum Pick up Without Affecting Embryological Parameters? A Retrospective Case-Matched Study.
作者信息
Paffoni Alessio, Ballabio Elisabetta, Cesana Sabrina, Ferrari Stefania, Wyssling Hilda, Bianchi Marco Claudio
机构信息
ASST Lariana, Infertility Unit, Cantù, Italy.
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
出版信息
J Reprod Infertil. 2019 Oct-Dec;20(4):209-217.
BACKGROUND
According to several laboratory protocols and specific conditions, fertilization (IVF) dishes with culture media can be prepared 24 in advance compared to routine protocols. However, it is not clear if this procedure can affect embryological outcomes.
METHODS
A nested case-control study was done in a cohort of couples undergoing IVF at the Infertility Unit of the ASST Lariana from August 2016 to July 2018. Cases were patients undergoing ovum pick up after a laboratory day off. Controls were patients undergoing ovum pick up after working days from Monday to Thursday. Culture media for oocyte culture and insemination were prepared about 42 and 18 before oocyte retrieval for cases and controls, respectively. Cases and controls were matched with a 1:2 ratio (for age, inseminated oocytes, length of stimulation). The "Good-Quality-Index" (GQI) was the main outcome to be compared between the two groups and was defined as good quality transferred or cryopreserved embryos on day 2 or 3+number of good quality blastocysts/inseminated oocytes.
RESULTS
A total of 76 cases and 152 matched controls were enrolled. The median GQI was equal to 33.0% (IQR: 20.0-50.0%) and 33.0% (IQR: 25.0-50.0%), in cases and controls, respectively (p=0.40). Study groups and GQI were not significantly correlated (correlation coefficient r=0.047, p=0.48). Main embryological parameters and cumulative pregnancy rates were similar between the two groups.
CONCLUSION
Our data support the vision that culture media can be prepared 24 in advance compared to routine protocols without affecting embryological outcomes.
背景
根据几种实验室方案和特定条件，与常规方案相比，含有培养基的体外受精（IVF）培养皿可提前24小时制备。然而，尚不清楚该操作是否会影响胚胎学结局。
方法
2016年8月至2018年7月，在ASST拉里亚纳不孕症治疗中心对一组接受IVF的夫妇进行了一项巢式病例对照研究。病例为在实验室休息日之后进行取卵的患者。对照为在周一至周四工作日之后进行取卵的患者。分别在病例组和对照组的卵母细胞采集前约42小时和18小时制备用于卵母细胞培养和授精的培养基。病例组和对照组按1:2的比例（年龄、授精卵母细胞数、刺激时间）进行匹配。“优质指数”（GQI）是两组之间要比较的主要结局指标，定义为第2天或第3天优质移植或冷冻保存胚胎数+优质囊胚数/授精卵母细胞数。
结果
共纳入76例病例和152例匹配对照。病例组和对照组的GQI中位数分别为33.0%（四分位间距：20.0 - 50.0%）和33.0%（四分位间距：25.0 - 50.0%）（p = 0.40）。研究组与GQI无显著相关性（相关系数r = 0.047，p = 0.48）。两组之间的主要胚胎学参数和累积妊娠率相似。
结论
我们的数据支持以下观点：与常规方案相比，培养基可提前24小时制备，且不影响胚胎学结局。
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