突尼斯人群中IGF1基因多态性与结肠癌风险

IGF1 polymorphisms and colon cancer risk in Tunisian population.

作者信息

Dhifallah Haifa, Aissi Sana, Njima Manel, Zakhama Abdelfatteh, Kenani Abderraouf

出版信息

Tunis Med. 2019 Dec;97(12):1407-1414.

Abstract

BACKGROUND

Insuline-like growth factor I (IGF1) is a peptide growth factor that promotes cell proliferation and inhibits apoptosis.

AIM

To examine the association of genetic variants in IGF1 (rs12423791, rs1019731, rs5742632, rs2033178 and rs2373722) with risk of colon cancer in Tunisia.

METHODS

The study included 76 formalin-fixed paraffin embedded primary colorectal carcinomas and paired normal colon. The five IGF1 polymorphisms were determined by polymerase chain-restriction fragment length polymorphism (PCR-RFLP).

RESULT

A significant differences in genotypes and alleles frequency of the five examined IGF1 polymorphisms was determined between tumor and healthy tissues of colon cancer patients (P<0,01). While, no significant association was found between genetic variation in IGF1 variants and clinic-pathological parameters in tumors tissues. Expect for rs2373722, a statistically significant correlation was detected between tumor localization and the presence of the (A) mutated allele (OR=0,49; 95% CI 0,25-0,99; P=0,03).

CONCLUSION

This analysis shows that IGF1gene polymorphisms rs12423791, rs1019731, rs5742632, rs2033178 and rs2373722 are associated with the risk of colon cancer in Tunisian population.

摘要

背景

胰岛素样生长因子I（IGF1）是一种促进细胞增殖并抑制细胞凋亡的肽生长因子。

目的

研究IGF1基因变异（rs12423791、rs1019731、rs5742632、rs2033178和rs2373722）与突尼斯结肠癌风险的关联。

方法

本研究纳入76例福尔马林固定石蜡包埋的原发性结直肠癌及配对的正常结肠组织。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测这5个IGF1基因多态性。

结果

在结肠癌患者的肿瘤组织与健康组织之间，所检测的5个IGF1基因多态性的基因型和等位基因频率存在显著差异（P<0.01）。然而，未发现IGF1基因变异与肿瘤组织临床病理参数之间存在显著关联。除rs2373722外，在肿瘤定位与（A）突变等位基因的存在之间检测到统计学显著相关性（OR=0.49；95%CI 0.25-0.99；P=0.03）。