天然化合物可减轻重金属诱导的 PC12 细胞损伤。
Natural compounds attenuate heavy metal-induced PC12 cell damage.
机构信息
Changchun Medical College, Changchun, China.
Hepatology Department, Jilin Hepatobiliary Hospital, Changchun, China.
出版信息
J Int Med Res. 2020 Jun;48(6):300060520930847. doi: 10.1177/0300060520930847.
OBJECTIVES
To investigate the neuroprotective effects of six natural compounds (caffeine, gallic acid, resveratrol, epigallocatechin gallate [EGCG], L-ascorbic acid and alpha tocopherol [Vitamin E] on heavy metal-induced cell damage in rat PC12 cells.
METHODS
In this experiment, rat PC12 cells were exposed to four heavy metals (CdCl, HgCl, CoCl and PbCl) at different concentrations and cell apoptosis, necrosis and oxidative stress were assessed with and without the addition of the six natural compounds.
RESULTS
The metals decreased cell viability but the natural compounds attenuated their effects on apoptosis, necrosis and reactive oxygen species (ROS) levels. Mitochondrial protein changes were involved in the regulation.
CONCLUSION
Overall, the natural compounds did provide protection against the metal-induced PC12 cell damage. These data suggest that natural compounds may have therapeutic potential against metal-induced neurodegenerative disease.
目的
研究六种天然化合物（咖啡因、没食子酸、白藜芦醇、表没食子儿茶素没食子酸酯[EGCG]、L-抗坏血酸和α生育酚[维生素 E]）对重金属诱导的大鼠 PC12 细胞损伤的神经保护作用。
方法
在这项实验中，将大鼠 PC12 细胞暴露于四种重金属（CdCl、HgCl、CoCl 和 PbCl）的不同浓度下，并在添加和不添加六种天然化合物的情况下评估细胞凋亡、坏死和氧化应激。
结果
这些金属降低了细胞活力，但天然化合物减轻了它们对细胞凋亡、坏死和活性氧（ROS）水平的影响。线粒体蛋白变化参与了调节。
结论
总的来说，天然化合物确实为重金属诱导的 PC12 细胞损伤提供了保护。这些数据表明，天然化合物可能对金属诱导的神经退行性疾病具有治疗潜力。
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