基于硼酸酯的灵敏荧光探针用于检测活细胞内源性过氧亚硝酸盐。
Boronate based sensitive fluorescent probe for the detection of endogenous peroxynitrite in living cells.
机构信息
Institute of Environmental Science, School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.
Institute of Environmental Science, School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Dec 15;243:118683. doi: 10.1016/j.saa.2020.118683. Epub 2020 Aug 5.
In this work, a novel boronate-based fluorescent probe (FAM) for the endogenous detection of peroxynitrite (ONOO) has been developed by using anthracycline as the fluorophore, arlyboronate as the recognition moiety, lipophilic cation as the mitochondrial targeting moiety. Upon reaction of the probe with ONOO, the oxidation and subsequent hydrolysis of ONOO to arlyboronate triggers quite rapid fluorescence off-on response, providing a sensitive and highly selective method for the detection of ONOO. In addition, probe holds high sensitivity with the detection limit of 3.2 nM and excellent specificity including a series of biologically relevant reactive oxygen species. Importantly, FAM with good water solubility displays excellent performances for imaging endogenous peroxynitrite produced by RAW264.7 cells.
在这项工作中，我们开发了一种新型的硼酸盐荧光探针（FAM），用于内源性检测过氧亚硝酸盐（ONOO）。该探针以蒽环类抗生素作为荧光团，硼酸酯作为识别部分，亲脂性阳离子作为线粒体靶向部分。当探针与 ONOO 反应时，ONOO 被氧化并随后水解为硼酸酯，触发了相当快速的荧光开-关响应，为 ONOO 的检测提供了一种灵敏且高选择性的方法。此外，该探针具有高灵敏度，检测限为 3.2 nM，并且具有出色的特异性，包括一系列与生物学相关的活性氧物质。重要的是，水溶性良好的 FAM 可以出色地对 RAW264.7 细胞产生的内源性过氧亚硝酸盐进行成像。
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