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Rad54 蛋白的分支迁移活性。

Branch Migration Activity of Rad54 Protein.

机构信息

Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA, USA.

出版信息

Methods Mol Biol. 2021;2153:145-167. doi: 10.1007/978-1-0716-0644-5_11.

DOI:10.1007/978-1-0716-0644-5_11
PMID:32840778
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8168686/
Abstract

Rad54 is a eukaryotic protein that plays an important role in homologous recombination. Rad54, a member of the Swi2/Snf2 family, binds to Holliday junctions with high specificity and promotes their branch migration in an ATP hydrolysis-dependent manner. Here we describe the methods our laboratory used to characterize the branch migration activity of Rad54. These assays are applicable for other branch migration proteins regardless of whether they have canonical helicase activity or not.

摘要

Rad54 是一种在真核生物中发挥重要作用的蛋白质，它参与同源重组。Rad54 是 Swi2/Snf2 家族的成员之一，它能与 Holliday 连接点特异性结合，并以依赖于 ATP 水解的方式促进其分支迁移。在这里，我们描述了实验室用来表征 Rad54 分支迁移活性的方法。这些测定方法适用于其他分支迁移蛋白，无论它们是否具有典型的解旋酶活性。

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本文引用的文献

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