在两个成熟阶段下，从 tempesquistle（Sideroxylon palmeri）果实中温和条件下提取的果胶的特性。
Characterization of pectin extracted under mild conditions from tempesquistle (Sideroxylon palmeri) fruit at two maturity stages.
机构信息
Unidad Tecnológica, Instituto Tecnológico Superior de Acatlán de Osorio, Puebla, México.
Instituto de Agroindustrias, Universidad Tecnológica de la Mixteca, Oaxaca, México.
出版信息
Acta Sci Pol Technol Aliment. 2020 Jul-Sep;19(3):347-357. doi: 10.17306/J.AFS.0850.
BACKGROUND
le tree (Sideroxylon palmeri) belongs to family Sapotaceae, and its fruits contain hydrophilic and hydrophobic gums. Aim of this study was to develop an extraction method for pectin, a hydrophilic gum, from green and ripe tempesquistle fruit. The extraction method was gentle to minimize potential structural changes in pectin, a maceration was used, and then pectin precipitation was induced with acetone at room temperature. Pectin characterization was carried out in two steps. The first step consisted of qualitative tests of Molisch, Fehling and Lugol. The second step consisted of quantitative tests to determine esterification degree (ED) by ATR-FTIR and D-galacturonic acid (D-GalA) content with a colorimetric assay. ATR-FTIR spectroscopic method revealed that green and ripe tempesquistle fruit have an ED of 0% and 30.4%, respectively. Both fruit have a low ED pectin. The content of D-GalA in green and ripe pectins was 18.8 ±2.7% and 20.2 ±0.6%, respectively. The yield obtained in green and ripe fruit samples was 1.6 ±0.2% and 3.0 ±0.1%, respectively. The extraction method allowed two types of pectins obtained in function of maturity stage of tempesquistle fruit distinguishable by.
METHODS
istle tree (Sideroxylon palmeri) belongs to family Sapotaceae, and its fruits contain hydrophilic and hydrophobic gums. Aim of this study was to develop an extraction method for pectin, a hydrophilic gum, from green and ripe tempesquistle fruit. The extraction method was gentle to minimize potential structural changes in pectin, a maceration was used, and then pectin precipitation was induced with acetone at room temperature. Pectin characterization was carried out in two steps. The first step consisted of qualitative tests of Molisch, Fehling and Lugol. The second step consisted of quantitative tests to determine esterification degree (ED) by ATR-FTIR and D-galacturonic acid (D-GalA) content with a colorimetric assay. ATR-FTIR spectroscopic method revealed that green and ripe tempesquistle fruit have an ED of 0% and 30.4%, respectively. Both fruit have a low ED pectin. The content of D-GalA in green and ripe pectins was 18.8 ±2.7% and 20.2 ±0.6%, respectively. The yield obtained in green and ripe fruit samples was 1.6 ±0.2% and 3.0 ±0.1%, respectively. The extraction method allowed two types of pectins obtained in function of maturity stage of tempesquistle fruit distinguishable by ATR.
RESULTS
istle tree (Sideroxylon palmeri) belongs to family Sapotaceae, and its fruits contain hydrophilic and hydrophobic gums. Aim of this study was to develop an extraction method for pectin, a hydrophilic gum, from green and ripe tempesquistle fruit. The extraction method was gentle to minimize potential structural changes in pectin, a maceration was used, and then pectin precipitation was induced with acetone at room temperature. Pectin characterization was carried out in two steps. The first step consisted of qualitative tests of Molisch, Fehling and Lugol. The second step consisted of quantitative tests to determine esterification degree (ED) by ATR-FTIR and D-galacturonic acid (D-GalA) content with a colorimetric assay. ATR-FTIR spectroscopic method revealed that green and ripe tempesquistle fruit have an ED of 0% and 30.4%, respectively. Both fruit have a low ED pectin. The content of D-GalA in green and ripe pectins was 18.8 ±2.7% and 20.2 ±0.6%, respectively. The yield obtained in green and ripe fruit samples was 1.6 ±0.2% and 3.0 ±0.1%, respectively. The extraction method allowed two types of pectins obtained in function of maturity stage of tempesquistle fruit distinguishable by ATR-FTIR.
背景
三叶柿（Sideroxylon palmeri）属于柿科，其果实含有亲水性和疏水性胶。本研究的目的是开发一种从绿色和成熟的 tempesquistle 果实中提取亲水性胶（果胶）的方法。该提取方法温和，最大限度地减少果胶潜在的结构变化，采用浸渍法，然后在室温下用丙酮诱导果胶沉淀。果胶的特征分析分两步进行。第一步包括 Molisch、Fehling 和 Lugol 的定性测试。第二步包括通过 ATR-FTIR 和比色法测定酯化度（ED）和 D-半乳糖醛酸（D-GalA）含量的定量测试。ATR-FTIR 光谱法表明，绿色和成熟的 tempesquistle 果实的 ED 分别为 0%和 30.4%。两种果实的 ED 果胶含量都较低。绿色和成熟果胶中的 D-GalA 含量分别为 18.8±2.7%和 20.2±0.6%。绿色和成熟果实样品的产率分别为 1.6±0.2%和 3.0±0.1%。该提取方法允许根据 tempesquistle 果实的成熟阶段获得两种类型的果胶，通过 ATR-FTIR 可以将其区分开来。
方法
三叶柿（Sideroxylon palmeri）属于柿科，其果实含有亲水性和疏水性胶。本研究的目的是开发一种从绿色和成熟的 tempesquistle 果实中提取亲水性胶（果胶）的方法。该提取方法温和，最大限度地减少果胶潜在的结构变化，采用浸渍法，然后在室温下用丙酮诱导果胶沉淀。果胶的特征分析分两步进行。第一步包括 Molisch、Fehling 和 Lugol 的定性测试。第二步包括通过 ATR-FTIR 和比色法测定酯化度（ED）和 D-半乳糖醛酸（D-GalA）含量的定量测试。ATR-FTIR 光谱法表明，绿色和成熟的 tempesquistle 果实的 ED 分别为 0%和 30.4%。两种果实的 ED 果胶含量都较低。绿色和成熟果胶中的 D-GalA 含量分别为 18.8±2.7%和 20.2±0.6%。绿色和成熟果实样品的产率分别为 1.6±0.2%和 3.0±0.1%。该提取方法允许根据 tempesquistle 果实的成熟阶段获得两种类型的果胶，通过 ATR-FTIR 可以将其区分开来。
结果
三叶柿（Sideroxylon palmeri）属于柿科，其果实含有亲水性和疏水性胶。本研究的目的是开发一种从绿色和成熟的 tempesquistle 果实中提取亲水性胶（果胶）的方法。该提取方法温和，最大限度地减少果胶潜在的结构变化，采用浸渍法，然后在室温下用丙酮诱导果胶沉淀。果胶的特征分析分两步进行。第一步包括 Molisch、Fehling 和 Lugol 的定性测试。第二步包括通过 ATR-FTIR 和比色法测定酯化度（ED）和 D-半乳糖醛酸（D-GalA）含量的定量测试。ATR-FTIR 光谱法表明，绿色和成熟的 tempesquistle 果实的 ED 分别为 0%和 30.4%。两种果实的 ED 果胶含量都较低。绿色和成熟果胶中的 D-GalA 含量分别为 18.8±2.7%和 20.2±0.6%。绿色和成熟果实样品的产率分别为 1.6±0.2%和 3.0±0.1%。该提取方法允许根据 tempesquistle 果实的成熟阶段获得两种类型的果胶，通过 ATR-FTIR 可以将其区分开来。
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