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sp. nov.,从水槽和健康绵羊的粪便中分离出来。

sp. nov., isolated from a water trough and the faeces of healthy sheep.

机构信息

Departamento Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, Valencia, Spain.

Institut Pasteur, Biology of Infection Unit, Paris, France.

出版信息

Int J Syst Evol Microbiol. 2020 Nov;70(11):5868-5879. doi: 10.1099/ijsem.0.004494.

Abstract

In the context of a study on the occurrence of species in an animal farm environment in Valencia, Spain, six -like isolates could not be assigned to any known species. Phylogenetic analysis based on the 16S rRNA gene and on 231 core genes grouped these isolates in a monophyletic clade within the genus , with highest similarity to . Whole-genome sequence analyses based on DNA-DNA hybridization, the average nucleotide blast and the pairwise amino acid identities against all currently known species confirmed that these isolates constituted a new taxon within the genus . Phenotypically, these isolates differed from other species mainly by the production of acid from inositol, the absence of acidification in presence of methyl α-d-glucoside, and the absence of α-mannosidase and nitrate reductase activities. The name sp. nov. is proposed for this novel species, and the type strain is CLIP 2019/00642 (=CIP 111799=DSM 110544).

摘要

在西班牙瓦伦西亚一个动物农场环境中 物种发生的研究背景下,有 6 株类似的分离株无法被分配到任何已知的物种。基于 16S rRNA 基因和 231 个核心基因的系统发育分析将这些分离株聚在一个单系分支内,与 最为相似。基于 DNA-DNA 杂交、平均核苷酸比对和与所有当前已知 物种的成对氨基酸同一性的全基因组序列分析证实,这些分离株构成了该属内的一个新分类群。表型上,这些分离株与其他 种主要区别在于能够从肌醇产酸、在存在甲基 α-d-葡糖苷时不酸化以及缺乏 α-甘露糖苷酶和硝酸盐还原酶活性。提议将这个新种命名为 sp. nov.,其模式菌株为 CLIP 2019/00642(=CIP 111799=DSM 110544)。

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