线粒体 RNA 修饰的质谱分析。
Mass Spectrometric Analysis of Mitochondrial RNA Modifications.
机构信息
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan.
出版信息
Methods Mol Biol. 2021;2192:89-101. doi: 10.1007/978-1-0716-0834-0_8.
PMID:33230768
Abstract
Mitochondrial RNAs are modified posttranscriptionally. These modifications are required for proper functioning of RNA molecules, and thereby contribute to essential mitochondrial processes. Herein, we describe our latest mass spectrometry-based platform for analysis of posttranscriptional modifications of mitochondrial tRNAs, and measuring the in vitro activity of mitochondrial RNA-modifying enzymes.
摘要
线粒体 RNA 是转录后修饰的。这些修饰对于 RNA 分子的正常功能是必需的，从而有助于基本的线粒体过程。本文中，我们描述了最新基于质谱的平台，用于分析线粒体 tRNA 的转录后修饰，并测量线粒体 RNA 修饰酶的体外活性。
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