基于谷胱甘肽-金/铂纳米簇过氧化物酶模拟物抑制的比色法测定半胱氨酸
Colorimetric determination of cysteine based on inhibition of GSH-Au/Pt NCs as peroxidase mimic.
作者信息
Li Jian-Jun, Qiao Dan, Yang Shou-Zhi, Weng Guo-Jun, Zhu Jian, Zhao Jun-Wu
机构信息
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Mar 5;248:119257. doi: 10.1016/j.saa.2020.119257. Epub 2020 Nov 28.
In this work, we reported a facile and highly sensitive strategy for colorimetric detection of cysteine (Cys) based on the inhibition of catalytic activity of bimetallic nanoclusters induced by Cys. Glutathione-modified gold-platinum nanoclusters (GSH-Au/Pt NCs) with different Au/Pt molar ratios were prepared via one-pot approach and utilized as peroxidase mimics to catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by HO. It has been found that Cys could inhibit the peroxidase-like activity of GSH-Au/Pt NCs efficiently, which leads to a decrease of the absorption intensity of the system at 652 nm with a fading of the blue color. These findings provide a worthy method for visualization and quantitative detection of Cys with different concentrations in the range from 0.5 to 30 μM, and the detection limit is 0.154 μM. Moreover, this method displays a promising application in colorimetric analysis of Cys in urine samples.
在本工作中，我们报道了一种基于半胱氨酸（Cys）对双金属纳米簇催化活性的抑制作用，用于比色检测Cys的简便且高灵敏度策略。通过一锅法制备了具有不同金/铂摩尔比的谷胱甘肽修饰的金-铂纳米簇（GSH-Au/Pt NCs），并将其用作过氧化物酶模拟物，以催化HO对3,3',5,5'-四甲基联苯胺（TMB）的氧化。研究发现，Cys能够有效抑制GSH-Au/Pt NCs的过氧化物酶样活性，这导致体系在652nm处的吸收强度降低，蓝色褪色。这些发现为可视化和定量检测浓度范围为0.5至30μM的不同浓度Cys提供了一种有价值的方法，检测限为0.154μM。此外，该方法在尿液样本中Cys的比色分析中显示出有前景的应用。
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