基于刃天青的针对……的药敏试验的开发与验证
Development and Validation of an Resazurin-Based Susceptibility Assay against .
作者信息
Abd Algaffar S O, Verbon A, van de Sande W W J, Khalid S A
机构信息
Faculty of Pharmacy, University of Science & Technology, Omdurman, Sudan.
Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands.
出版信息
Antimicrob Agents Chemother. 2021 Feb 17;65(3). doi: 10.1128/AAC.01338-20.
We present an susceptibility assay for hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin. Results were comparable with those of a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2-tetrazolium-5-carboxanilide salt (XTT)-based susceptibility assay. The lowest MICs were obtained for itraconazole and posaconazole (MIC, 0.016 µg/ml) followed by voriconazole (MIC, 0.063 µg/ml). Amphotericin B, micafungin, and terbinafine appeared much less effective.
我们介绍了一种使用刃天青进行终点读数的菌丝体药敏试验。使用该试验，两性霉素B、伊曲康唑、伏立康唑、泊沙康唑、特比萘芬和米卡芬净获得了可重复的最低抑菌浓度（MIC）。结果与基于2,3-双-(2-甲氧基-4-硝基-5-磺基苯基)-2-四唑-5-羧基苯胺盐（XTT）的药敏试验结果相当。伊曲康唑和泊沙康唑的最低MIC值最低（MIC，0.016 µg/ml），其次是伏立康唑（MIC，0.063 µg/ml）。两性霉素B、米卡芬净和特比萘芬的效果要差得多。
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