2008年至2019年期间在日本分离出的传染性支气管炎病毒的基因分型
Genotyping of infectious bronchitis viruses isolated in Japan during 2008-2019.
作者信息
Mase Masaji, Gotou Makiko, Inoue Daisuke, Watanabe Satoko, Iseki Hiroshi
机构信息
Division of Viral Disease and Epidemiology, National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
出版信息
J Vet Med Sci. 2021 Apr 3;83(3):522-526. doi: 10.1292/jvms.20-0620. Epub 2021 Jan 12.
Seventeen isolates of infectious bronchitis virus (IBV) were obtained from various prefectures of Japan during 2008-2019 and genetically analyzed. The IBV isolates were classified into six genetic groups, based on phylogenetic analysis of the S1 gene. The S1 genotypes were distinguishable by a newly developed restriction fragment length polymorphism (RFLP) method using three endonucleases, Hae II, Hpa I, and Fok I. Moreover, the isolates were classified into four genetic groups, based on phylogenetic analysis of the S2 gene. However, novel genetic groups based on a combination of S1 and S2 genotypes, which were undetected previously, were confirmed in this study, indicating that various recombinant IBV strains were prevalent in poultry in Japan.
2008年至2019年期间，从日本各府收集了17株传染性支气管炎病毒（IBV）并进行基因分析。基于S1基因的系统发育分析，这些IBV分离株被分为六个遗传组。使用三种核酸内切酶Hae II、Hpa I和Fok I，通过新开发的限制性片段长度多态性（RFLP）方法可区分S1基因型。此外，基于S2基因的系统发育分析，这些分离株被分为四个遗传组。然而，本研究证实了基于S1和S2基因型组合的新型遗传组，这些遗传组以前未被检测到，这表明日本家禽中存在各种重组IBV毒株。
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