一种基于肽的酶联免疫吸附测定法用于检测抗禽传染性支气管炎病毒抗体

A Peptide-Based Enzyme-Linked Immunosorbent Assay for Detecting Antibodies Against Avian Infectious Bronchitis Virus.

作者信息

Yin Liping, Wu Qi, Lin Zhixian, Qian Kun, Shao Hongxia, Wan Zhimin, Liu Yuelong, Ye Jianqiang, Qin Aijian

机构信息

Key Laboratory of Avian Preventive Medicine, Ministry of Education, Yangzhou University, Yangzhou, China.

JiangsuLihua Animal Husbandry Co., Ltd, Changzhou, China.

出版信息

Front Vet Sci. 2021 Jan 21;7:619601. doi: 10.3389/fvets.2020.619601. eCollection 2020.

DOI:10.3389/fvets.2020.619601

PMID:33553284

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7859331/

Abstract

Infectious bronchitis virus (IBV) causes substantial loss to the poultry industry despite extensive vaccination. Assessing the antibody response is important for the development and evaluation of effective vaccines. We have developed an enzyme-linked immunosorbent assay (ELISA) for the detection of IBV-specific antibodies, using a synthetic peptide based on a conserved sequence in the IBV spike protein. This peptide-based ELISA (pELISA) specifically detects antibodies to different genotypes of IBV but not antibodies against other common chicken viruses. This assay could detect IBV-specific antibody response on as early as day 7 postinfection. In the testing with field serum samples collected from chickens administered with IBV vaccines, the sensitivity, specificity, and accuracy of pELISA were 98.30, 94.12, and 98.8%, respectively, relative to indirect immunofluorescence assay. Our data demonstrate that the pELISA is of value for the detection of IBV antibody and the evaluation of IBV vaccines.

摘要

尽管进行了广泛的疫苗接种，但传染性支气管炎病毒（IBV）仍给家禽业造成了巨大损失。评估抗体反应对于有效疫苗的研发和评估至关重要。我们开发了一种酶联免疫吸附测定（ELISA）方法，用于检测IBV特异性抗体，该方法使用基于IBV刺突蛋白保守序列的合成肽。这种基于肽的ELISA（pELISA）能够特异性检测针对不同基因型IBV的抗体，但不能检测针对其他常见鸡病毒的抗体。该检测方法最早可在感染后第7天检测到IBV特异性抗体反应。在用接种了IBV疫苗的鸡采集的现场血清样本进行检测时，相对于间接免疫荧光测定，pELISA的灵敏度、特异性和准确性分别为98.30%、94.12%和98.8%。我们的数据表明，pELISA在检测IBV抗体和评估IBV疫苗方面具有价值。