基于苯甲酰肼衍生物的镁选择性探针的合成与表征及其在细胞成像中的应用。
Synthesis and Characterization of a Mg-Selective Probe Based on Benzoyl Hydrazine Derivative and Its Application in Cell Imaging.
机构信息
Laboratory of Environmental Monitoring, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571101, China.
Laboratory of Tropical Biomedicine and Biotechnology, Hainan Medical University, Haikou 571101, China.
出版信息
Molecules. 2021 Apr 23;26(9):2457. doi: 10.3390/molecules26092457.
A simple benzoyl hydrazine derivative was successfully synthesized and characterized as Mg-selective fluorescent probe. The binding of with Mg caused an obvious fluorescence enhancement at 482 nm. The fluorescent, UV-vis spectra, H-NMR, and IR spectra confirmed the formation of -Mg complex, and the formation of a 1:1 stoichiometry complex was proved by Job's plot and mass spectrometry. The recognition mechanism of to Mg was owing to the photoinduced electron transfer effect (PET). The fluorescent response was linear in the range of 0.9-4.0 µM with the detection limit of 0.3 µM Mg in water-ethanol solution (1:9, :, pH10.0, 20 mM HEPES). In addition, the results of cell imaging of Mg in Hl-7701 cells was satisfying.
一种简单的苯甲酰肼衍生物被成功合成并被表征为 Mg 选择性荧光探针。与 Mg 的结合导致在 482nm 处出现明显的荧光增强。荧光、紫外-可见光谱、H-NMR 和 IR 光谱证实了-Mg 配合物的形成，通过 Job 图和质谱证明了 1:1 化学计量比配合物的形成。对 Mg 的识别机制归因于光诱导电子转移效应（PET）。荧光响应在 0.9-4.0µM 范围内呈线性，在水-乙醇溶液（1:9，:，pH10.0，20mM HEPES）中检测限为 0.3µM Mg。此外，在 Hl-7701 细胞中对 Mg 的细胞成像结果令人满意。
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