家禽中重组H5N1和O157联合灭活疫苗的制备与评价试验。

Trials for preparation and evaluation of a combined inactivated reassorted H5N1 and O157 vaccine in poultry.

作者信息

Sayed Marwa Fathy El, Soliman Reem A, Ghanem Heba Mohamed, Khedr Marwa M S, Mohamed Gina M, Safty Mounir Mohamed Diab El

机构信息

Central Laboratory for Evaluation of Veterinary Biologics, Agricultural Research Center, Giza, Egypt.

出版信息

Vet World. 2021 Jun;14(6):1677-1681. doi: 10.14202/vetworld.2021.1677-1681. Epub 2021 Jun 28.

DOI:10.14202/vetworld.2021.1677-1681

PMID:34316218

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8304417/

Abstract

BACKGROUND AND AIM

Avian influenza (AI), which is one of the major respiratory diseases of poultry, and () have caused major economic losses around the world, including in Egypt. Therefore, in this study, we aimed to produce a vaccine from O157 and AI H5N1 formulated with Montanide ISA70 for the protection of poultry against both diseases.

MATERIALS AND METHODS

We divided one hundred 3-week-old chicks into four groups: Group 1 was vaccinated with prepared inactivated AI H5N1formulated with Montanide ISA70, Group 2 was vaccinated with inactivated formulated with Montanide ISA70, Group 3 was vaccinated with combined and AI H5N1 formulated with Montanide ISA70, and Group 4 was an unvaccinated control group. We measured the immune response using the HI (hemagglutination inhibition) test, enzyme-linked immunosorbent assay (ELISA), and the challenge test.

RESULTS

We found the three vaccines to be safe and sterile during all periods of examination and observation. The HI test showed that Group 1 exhibited specific antibody titers of 2.3 log, 4.3 log, 7.5 log, 7.8 log, 8 log, and 8.1 log from week 2 to week 7, respectively, post-vaccination. Group 3 exhibited antibody titers of 3.3 log, 5.8 log, 7.8 log, 8 log, 8.3 log, and 8.3 log from week 2 to week 7, respectively, post-vaccination. The immune response in both groups reached a high titer at week 6. The combined inactivated and AI H5N1 vaccine generated a higher immune response than the inactivated AI H5N1 vaccine, and a significant difference exists between the two groups. For Groups 2 and 3, the ELISA antibody titer exhibited its lowest value, 1996.5 and 2036.7, respectively, at week 1 post-vaccination; whereas, both groups exhibited the highest titers, 2227.7 (for Group 2) and 2287.3 (for Group 3), in week 3 post-booster. The ELISA for the combined inactivated and AI H5N1 vaccine had a higher titer than did the inactivated vaccine, and a significant difference exists between the two groups. Moreover, the protection rate was higher in Group 3, with 100% for and 90% for the AI H5N1 vaccine.

CONCLUSION

Our findings demonstrate that producing a combined vaccine using and AI H5N1 formulated with Montanide ISA70 is recommended for protection against both diseases.

摘要

背景与目的

禽流感（AI）是家禽的主要呼吸道疾病之一，与（此处原文缺失部分内容）在包括埃及在内的世界各地都造成了重大经济损失。因此，在本研究中，我们旨在制备一种由O157和AI H5N1与Montanide ISA70佐剂配制而成的疫苗，用于保护家禽免受这两种疾病的侵害。

材料与方法

我们将100只3周龄的雏鸡分为四组：第1组用与Montanide ISA70佐剂配制的制备好的灭活AI H5N1疫苗接种；第2组用与Montanide ISA70佐剂配制的灭活（此处原文缺失部分内容）疫苗接种；第3组用与Montanide ISA70佐剂配制的（此处原文缺失部分内容）和AI H5N1联合疫苗接种；第4组为未接种的对照组。我们使用血凝抑制（HI）试验、酶联免疫吸附测定（ELISA）和攻毒试验来测量免疫反应。

结果

在所有检查和观察期间，我们发现这三种疫苗都是安全且无菌的。HI试验表明，接种疫苗后第2周至第7周，第1组的特异性抗体效价分别为2.3 log、4.3 log、7.5 log、7.8 log、8 log和8.1 log。接种疫苗后第2周至第7周，第3组的抗体效价分别为3.3 log、5.8 log、7.8 log、8 log、8.3 log和8.3 log。两组的免疫反应在第6周达到高滴度。（此处原文缺失部分内容）和AI H5N1联合灭活疫苗产生的免疫反应高于灭活AI H5N1疫苗，两组之间存在显著差异。对于第2组和第3组，ELISA抗体效价在接种疫苗后第1周分别达到最低值1996.5和2036.7；而在加强免疫后第3周，两组均表现出最高效价，第2组为2227.7，第3组为2287.3。（此处原文缺失部分内容）和AI H5N1联合灭活疫苗的ELISA效价比灭活（此处原文缺失部分内容）疫苗更高，两组之间存在显著差异。此外，第3组的保护率更高，（此处原文缺失部分内容）疫苗的保护率为100%，AI H5N1疫苗的保护率为90%。