基于 BODIPY 的半乳糖苷酶激活型荧光探针用于细菌检测。
A Galactosidase-Activatable Fluorescent Probe for Detection of Bacteria Based on BODIPY.
机构信息
College of Chemistry, Nanchang University, Nanchang 330031, China.
Key Laboratory of Organic Chemistry in Jiangxi Province, Institute of Organic Chemistry, Jiangxi Science & Technology Normal University, Nanchang 330013, China.
出版信息
Molecules. 2021 Oct 8;26(19):6072. doi: 10.3390/molecules26196072.
Pathogenic infection is one of the most widespread foodborne diseases, so the development of sensitive, reliable and easy operating detection tests is a key issue for food safety. Identifying bacteria with a fluorescent medium is more sensitive and faster than using chromogenic media. This study designed and synthesized a -galactosidase-activatable fluorescent probe BOD-Gal for the sensitive detection of . It employed a biocompatible and photostable 4,4-difluoro-3a,4a-diaza-s-indancene (BODIPY) as the fluorophore to form a --glycosidic bond with galactose, allowing the BOD-Gal to show significant on-off fluorescent signals for in vitro and in vivo bacterial detection. This work shows the potential for the use of a BODIPY based enzyme substrate for pathogen detection.
致病性感染是最广泛的食源性疾病之一，因此开发敏感、可靠和易于操作的检测试验是食品安全的关键问题。使用荧光培养基比使用显色培养基鉴定细菌更敏感、更快。本研究设计并合成了一种β-半乳糖苷酶激活型荧光探针 BOD-Gal，用于灵敏检测。它采用了生物相容性和光稳定性好的 4,4-二氟-3a,4a-二氮杂-s-茚满（BODIPY）作为荧光团，与半乳糖形成 --糖苷键，使 BOD-Gal 能够在体外和体内细菌检测中显示出显著的开-关荧光信号。这项工作表明，基于 BODIPY 的酶底物在病原体检测方面具有应用潜力。
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