长链非编码 RNA HOXC-AS3 通过海绵吸附 miR-96 促进非小细胞肺癌细胞迁移和侵袭。
LncRNA HOXC-AS3 increases non-small cell lung cancer cell migration and invasion by sponging premature miR-96.
机构信息
Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan City, People's Republic of China.
出版信息
Expert Rev Respir Med. 2022 May;16(5):587-593. doi: 10.1080/17476348.2022.2030223. Epub 2022 Jan 27.
BACKGROUND
Long noncoding RNA (lncRNA) HOXC cluster antisense RNA 3 (HOXC-AS3) has been involved in breast cancer and gastric cancer, while its role in non-small cell lung cancer (NSCLC) is unknown.
METHODS
The expression of HOXC-AS3 and miR-96 (both mature and premature) were detected using RT-qPCR. Nuclear fractionation assay and RNA pull-down assay were performed to detect the subcellular location of HOXC-AS3 and potential interaction with premature miR-96, respectively. Overexpression assays were performed to determine the role of HOXC-AS3 in the maturation of miR-96. Transwell assays were performed to explore the role of HOXC-AS3 and miR-96 in NSCLC cell invasion and migration.
RESULTS
NSCLC tissues exhibited significantly increased expression levels of HOXC-AS3 and premature miR-96. HOXC-AS3 was localized to both nucleus and cytoplasm, and a direct interaction between HOXC-AS3 and premature miR-96 was observed. In NSCLC cells, HOXC-AS3 upregulated the expression of premature miR-96 but downregulated the expression of mature miR-96. Moreover, HOXC-AS3 suppressed the role of miR-96 in inhibiting NSCLC cell invasion and migration.
CONCLUSION
HOXC-AS3 may increase NSCLC cell growth and invasion by sponging premature miR-96 to suppress its maturation.
背景
长链非编码 RNA（lncRNA）HOXC 簇反义 RNA3（HOXC-AS3）已参与乳腺癌和胃癌的发生，但其在非小细胞肺癌（NSCLC）中的作用尚不清楚。
方法
采用 RT-qPCR 检测 HOXC-AS3 和 miR-96（成熟体和前体）的表达。采用核分离实验和 RNA 下拉实验分别检测 HOXC-AS3 的亚细胞定位及其与前体 miR-96 的潜在相互作用。过表达实验确定 HOXC-AS3 在前体 miR-96 成熟过程中的作用。Transwell 实验探讨 HOXC-AS3 和 miR-96 在 NSCLC 细胞侵袭和迁移中的作用。
结果
NSCLC 组织中 HOXC-AS3 和前体 miR-96 的表达水平显著升高。HOXC-AS3 定位于细胞核和细胞质，并且观察到 HOXC-AS3 与前体 miR-96 之间的直接相互作用。在 NSCLC 细胞中，HOXC-AS3 上调前体 miR-96 的表达，但下调成熟 miR-96 的表达。此外，HOXC-AS3 抑制了 miR-96 抑制 NSCLC 细胞侵袭和迁移的作用。
结论
HOXC-AS3 可能通过海绵吸附前体 miR-96 来抑制其成熟，从而增加 NSCLC 细胞的生长和侵袭。
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