富含海洋油的补充剂可增加肥胖成年人特定的血浆特异性促解决介质。
Enriched Marine Oil Supplement Increases Specific Plasma Specialized Pro-Resolving Mediators in Adults with Obesity.
机构信息
Department of Nutrition, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
The University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC, USA.
出版信息
J Nutr. 2022 Jul 6;152(7):1783-1791. doi: 10.1093/jn/nxac075.
BACKGROUND
Specialized pro-resolving mediators (SPMs), synthesized from PUFAs, resolve inflammation and return damaged tissue to homeostasis. Thus, increasing metabolites of the SPM biosynthetic pathway may have potential health benefits for select clinical populations, such as subjects with obesity who display dysregulation of SPM metabolism. However, the concentrations of SPMs and their metabolic intermediates in humans with obesity remains unclear.
OBJECTIVES
The primary objective of this study was to determine if a marine oil supplement increased specific metabolites of the SPM biosynthetic pathway in adults with obesity. The second objective was to determine if the supplement changed the relative abundance of key immune cell populations. Finally, given the critical role of antibodies in inflammation, we determined if ex vivo CD19 + B-cell antibody production was modified by marine oil intervention.
METHODS
Twenty-three subjects [median age: 56 y; BMI (in kg/m2): 33.1] consumed 2 g/d of a marine oil supplement for 28-30 d. The supplement was particularly enriched with 18-hydroxyeicosapentaenoic (HEPE), 14-hydroxydocosahexaenoic acid (14-HDHA), and 17-HDHA. Blood was collected pre- and postsupplementation for plasma mass spectrometry oxylipin and fatty acid analyses, flow cytometry, and B-cell isolation. Paired t-tests and Wilcoxon tests were used for statistical analyses.
RESULTS
Relative to preintervention, the supplement increased 6 different HEPEs and HDHAs accompanied by changes in plasma PUFAs. Resolvin E1 and docosapentaenoic acid-derived maresin 1 concentrations were increased 3.5- and 4.7-fold upon intervention, respectively. The supplement did not increase the concentration of D-series resolvins and had no effect on the abundance of immune cells. Ex vivo B-cell IgG but not IgM concentrations were lowered postsupplementation.
CONCLUSIONS
A marine oil supplement increased select SPMs and their metabolic intermediates in adults with obesity. Additional studies are needed to determine if increased concentrations of specific SPMs control the resolution of inflammation in humans with obesity. This trial was registered at clinicaltrials.gov as NCT04701138.
背景
特殊的促解决介质（SPM），由多不饱和脂肪酸合成，可缓解炎症并使受损组织恢复到体内平衡。因此，增加 SPM 生物合成途径的代谢物可能对某些临床人群具有潜在的健康益处，例如肥胖人群，他们的 SPM 代谢失调。然而，肥胖人群中 SPM 及其代谢中间产物的浓度尚不清楚。
目的
本研究的主要目的是确定海洋油补充剂是否会增加肥胖成年人 SPM 生物合成途径的特定代谢物。第二个目的是确定补充剂是否会改变关键免疫细胞群体的相对丰度。最后，鉴于抗体在炎症中的关键作用，我们确定海洋油干预是否会改变体外 CD19+B 细胞抗体的产生。
方法
23 名受试者[中位数年龄：56 岁；BMI（kg/m2）：33.1] 每天服用 2 克海洋油补充剂 28-30 天。该补充剂特别富含 18-羟基二十碳五烯酸（HEPE），14-羟基二十二碳六烯酸（14-HDHA）和 17-羟基二十二碳六烯酸（17-HDHA）。在补充前后采集血液，用于血浆质谱法氧化脂和脂肪酸分析、流式细胞术和 B 细胞分离。使用配对 t 检验和 Wilcoxon 检验进行统计分析。
结果
与干预前相比，补充剂增加了 6 种不同的 HEPE 和 HDHA，同时还改变了血浆多不饱和脂肪酸。干预后，内源性 resolvin E1 和 docosapentaenoic 酸衍生的maresin 1 浓度分别增加了 3.5 倍和 4.7 倍。补充剂并未增加 D 系列 resolvin 的浓度，也对免疫细胞的丰度没有影响。体外 B 细胞 IgG 浓度但不是 IgM 浓度在补充后降低。
结论
海洋油补充剂可增加肥胖成年人中特定的 SPM 及其代谢中间产物。需要进一步的研究来确定特定 SPM 浓度的增加是否可以控制肥胖人群的炎症消退。本试验在 clinicaltrials.gov 上注册为 NCT04701138。
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