遗传编码脂质生物传感器的最新进展。
An update on genetically encoded lipid biosensors.
机构信息
Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261.
出版信息
Mol Biol Cell. 2022 May 1;33(5). doi: 10.1091/mbc.E21-07-0363.
Abstract
Specific lipid species play central roles in cell biology. Their presence or enrichment in individual membranes can control properties or direct protein localization and/or activity. Therefore, probes to detect and observe these lipids in intact cells are essential tools in the cell biologist's freezer box. Herein, we discuss genetically encoded lipid biosensors, which can be expressed as fluorescent protein fusions to track lipids in living cells. We provide a state-of-the-art list of the most widely available and reliable biosensors and highlight new probes (circa 2018-2021). Notably, we focus on advances in biosensors for phosphatidylinositol, phosphatidic acid, and PI 3-kinase lipid products.
摘要
特定的脂质种类在细胞生物学中起着核心作用。它们在单个膜中的存在或富集可以控制性质或指导蛋白质的定位和/或活性。因此，用于在完整细胞中检测和观察这些脂质的探针是细胞生物学家冷冻箱中的基本工具。在这里，我们讨论了遗传编码的脂质生物传感器，它可以作为荧光蛋白融合来追踪活细胞中的脂质。我们提供了最广泛使用和可靠的生物传感器的最新清单，并突出了新的探针（约 2018-2021 年）。值得注意的是，我们专注于磷脂酰肌醇、磷脂酸和 PI 3-激酶脂质产物的生物传感器的进展。
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