Probiotic-Mediated Biosynthesis of Silver Nanoparticles and Their Antibacterial Applications against Pathogenic Strains of O157:H7.

The present study aimed to suggest a simple and environmentally friendly biosynthesis method of silver nanoparticles (AgNPs) using the strain MAHUQ-74 isolated from kimchi. Antibacterial activity and mechanisms of AgNPs against antibiotic-resistant pathogenic strains of O157:H7 were investigated. The strain MAHUQ-74 had 99.93% relatedness to the NBRC 101234 strain. The biosynthesized AgNPs had a strong surface plasmon resonance (SPR) peak at 430 nm. The transmission electron microscope (TEM) image shows the spherical shape and size of the synthesized AgNPs is 13 to 50 nm. XRD analysis and SAED pattern revealed the crystal structure of biosynthesized AgNPs. Fourier transform infrared spectroscopy (FTIR) data showed various functional groups associated with the reduction of silver ions to AgNPs. The resultant AgNPs showed strong antibacterial activity against nine O157:H7 pathogens. Minimum inhibitory concentration (MIC) values of the AgNPs synthesized by strain MAHUQ-74 were 3.12 μg/mL for eight O157:H7 strains and 12.5 μg/mL for strain ATCC 25922. Minimum bactericidal concentrations (MBCs) were 25 μg/mL for O157:H7 ATCC 35150, O157:H7 ATCC 43895, O157:H7 ATCC 43890, O157:H7 ATCC 43889, and ATCC 25922; and 50 μg/mL for O157:H7 2257, O157: NM 3204-92, O157:H7 8624 and O157:H7 ATCC 43894. FE-SEM analysis demonstrated that the probiotic-mediated synthesized AgNPs produced structural and morphological changes and destroyed the membrane integrity of pathogenic O157:H7. Therefore, AgNPs synthesized by strain MAHUQ-74 may be potential antibacterial agents for the control of antibiotic-resistant pathogenic strains of O157:H7.