Bacteriostatic effects of phage F23s1 and its endolysin on Vibrio parahaemolyticus.

Vibrio parahaemolyticus is a common foodborne pathogenic bacterium and drug-resistant strains are now widespread. Phages led by drug-resistant V. parahaemolyticus strains are promising means to decrease the pressure on public health. We isolated a V. parahaemolyticus-specific bacteriophage F23s1 that was active at wide ranges of temperature (30-60°C) and pH (4-10). Phage F23s1 exhibited a specific host range; in that, only 13 of the 23 V. parahaemolyticus strains were lysed. F23s1 effectively inhibited the growth of V. parahaemolyticus strain F23 in shrimp at 25°C within 12 h at a multiplicity of infection of 1000. We sequenced the genome of phage F23s1 which comprised a 76,648-bp DNA with 105 open reading frames (ORFs) and identified an endolysin gene ORF52 that was then cloned and successfully expressed in Escherichia coli. The recombinant ORF52 protein significantly decreased OD of V. parahaemolyticus F23 from 0.978 to 0.249 when used at 20 µmol/L within 60 min. The endolysin also showed lytic activity against a panel of 23 drug-resistant V. parahaemolyticus and 12 Salmonella strains with a higher lytic ability for V. parahaemolyticus. The phage F23s1 and its endolysin will be useful for preventing and controlling V. parahaemolyticus in food safety.