白细胞介素 9 及其受体在间质性肺疾病患者支气管肺泡灌洗液淋巴细胞中的表达。

IL-9 and IL-9 receptor expression in lymphocytes from bronchoalveolar lavage fluid of patients with interstitial lung disease.

机构信息

Center for Interstitial and Rare Lung Disease, Department of Pneumology, Ruhrlandklinik University Hospital, University of Duisburg-Essen, Essen, Germany.

Institute of Pathology, University Hospital Essen, Essen, Germany.

出版信息

Immunobiology. 2022 Sep;227(5):152258. doi: 10.1016/j.imbio.2022.152258. Epub 2022 Aug 12.

DOI:10.1016/j.imbio.2022.152258

PMID:35998415

Abstract

INTRODUCTION

IL-9, mainly produced by T helper 9 (Th9) cells, promotes allergic airway inflammation and remodeling through the interaction with its receptor (IL-9R). Th9 cells and IL-9 have also been implicated in tissue fibrosis and autoimmunity pathways. However, the role of IL-9/IL-9R in the pathogenesis of interstitial lung disease (ILD) is unknown.

AIM

To evaluate IL-9/IL-9R expression in bronchoalveolar lavage fluid (BALF) lymphocytes of patients with various ILDs.

METHODS

Consecutive patients with ILD, who underwent BAL for diagnostic purposes, were studied. As control group, consecutive patients without evidence of ILD were included. Immunocytochemical staining of BALF lymphocytes for IL-9 and IL-9R was performed and evaluated by two independent readers.

RESULTS

45 patients, of them 8 had idiopathic pulmonary fibrosis (IPF), 12 nonspecific interstitial pneumonia (NSIP), 10 sarcoidosis, 9 hypersensitivity pneumonitis (HP), 6 cryptogenic organizing pneumonia (COP), and 24 controls were studied. In the ILD group, the highest BALF lymphocyte count was seen in HP followed by NSIP, COP, sarcoidosis, and IPF (p < 0.05 for HP vs IPF). The highest percentages of IL-9 and IL-9R positive lymphocytes were seen in COP. Conversely, NSIP showed the lowest rate of IL-9, and sarcoidosis the lowest rate of IL-9R positive lymphocytes. Only in NSIP, a direct correlation between IL and 9 and IL-9R positive lymphocytes was seen (r = 0.639, p = 0.025).

CONCLUSION

BALF lymphocytes IL-9 and IL-9R expression differs between various ILDs and could reflect different pathogenetic mechanisms.

摘要

简介

白细胞介素 9（IL-9）主要由辅助性 T 细胞 9（Th9）细胞产生，通过与其受体（IL-9R）相互作用促进过敏性气道炎症和重塑。Th9 细胞和 IL-9 也参与组织纤维化和自身免疫途径。然而，IL-9/IL-9R 在间质性肺病（ILD）发病机制中的作用尚不清楚。

目的

评估各种 ILD 患者支气管肺泡灌洗液（BALF）淋巴细胞中 IL-9/IL-9R 的表达。

方法

连续纳入因诊断目的而行 BAL 的 ILD 患者进行研究。连续纳入无 ILD 证据的患者作为对照组。对 BALF 淋巴细胞进行 IL-9 和 IL-9R 的免疫细胞化学染色，并由两名独立的观察者进行评估。

结果

共纳入 45 例患者，其中 8 例为特发性肺纤维化（IPF），12 例为非特异性间质性肺炎（NSIP），10 例为结节病，9 例为过敏性肺炎（HP），6 例为隐源性机化性肺炎（COP），24 例为对照组。在 ILD 组中，HP 患者的 BALF 淋巴细胞计数最高，其次是 NSIP、COP、结节病和 IPF（HP 与 IPF 相比，p<0.05）。COP 患者的 IL-9 和 IL-9R 阳性淋巴细胞比例最高。相反，NSIP 患者的 IL-9 比例最低，结节病患者的 IL-9R 阳性淋巴细胞比例最低。仅在 NSIP 中，IL-9 和 IL-9R 阳性淋巴细胞之间存在直接相关性（r=0.639，p=0.025）。