新种，新属，新种，新种，四个来自无刺蜜蜂的新的乳酸菌分离株和 。
sp. nov., gen. nov. sp. nov., sp. nov. and sp. nov., four new lactic acid bacterial isolates from stingless bees and .
机构信息
Department of Wine Science, School of Agriculture, Food & Wine, The University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia.
South Australian Genomics Centre (SAGC), SAHMRI, Adelaide, SA 5000, Australia.
出版信息
Int J Syst Evol Microbiol. 2022 Sep;72(9). doi: 10.1099/ijsem.0.005588.
Four strains, SG5_A10, SGEP1_A5, SG4_D2, and SG4_A1, were isolated from the honey or homogenate of Australian stingless bee species and . Based on 16S rRNA gene phylogeny, core gene phylogenetics, whole genome analyses such as determination of amino acid identity (AAI), cAAI of conserved genes, average nucleotide identity (ANI), and digital DNA-DNA hybridization (dDDH), chemotaxonomic analyses, and the novel isolation sources and unique geography, we propose three new species and one genus with the names sp. nov. (SG5_A10 = LMG 32133 = NBRC 114991), sp. nov. (SG4_A1 = LMG 32125 = NBRC 114984), sp. nov. (SG4_D2 = LMG 32126 = NBRC 115004) and sp. nov. (SGEP1_A5 = LMG 32134 = NBRC 114992). Three out of the four strains were found to be fructophilic, where SG5_A10 and SGEP1_A5 belong to obligately fructophilic lactic acid bacteria, and SG4_D2 representing a new type denoted here as kinetically fructophilic. This study represents the first published lactic acid bacterial species associated with the unique niche of Australian stingless bees.
从澳大利亚无刺蜜蜂的蜂蜜或匀浆中分离出了四个菌株，分别为 SG5_A10、SGEP1_A5、SG4_D2 和 SG4_A1。基于 16S rRNA 基因系统发育、核心基因系统发育、全基因组分析（如氨基酸同一性 (AAI)、保守基因的 cAAI、平均核苷酸同一性 (ANI) 和数字 DNA-DNA 杂交 (dDDH) 的测定、化学生态分析以及新的分离源和独特的地理位置，我们提出了三个新种和一个新属，分别命名为 sp. nov.（SG5_A10 = LMG 32133 = NBRC 114991）、 sp. nov.（SG4_A1 = LMG 32125 = NBRC 114984）、 sp. nov.（SG4_D2 = LMG 32126 = NBRC 115004）和 sp. nov.（SGEP1_A5 = LMG 32134 = NBRC 114992）。这四个菌株中有三个是果糖嗜性的，其中 SG5_A10 和 SGEP1_A5 属于专性果糖嗜性乳酸菌，而 SG4_D2 代表一种新的类型，在此表示为动力学果糖嗜性。这项研究代表了首次报道与澳大利亚无刺蜜蜂独特生态位相关的乳酸菌物种。
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