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干燥策略对含有的微生物生态制剂的孢子形成和效价的影响 。 你提供的原文似乎不太完整,最后的“with.”后面应该还有内容。

Effects of drying strategies on sporulation and titer of microbial ecological agents with .

作者信息

Li Chonglei, Zhao Kai, Ma Litong, Zhao Ji, Zhao Zhi-Min

机构信息

Inner Mongolia Key Laboratory of Environmental Pollution Control and Wastes Reuse, School of Ecology and Environment, Inner Mongolia University, Hohhot, China.

Key Laboratory of Ecology and Resource Use of the Mongolian Plateau (Ministry of Education), Inner Mongolia University, Hohhot, China.

出版信息

Front Nutr. 2022 Sep 27;9:1025248. doi: 10.3389/fnut.2022.1025248. eCollection 2022.

Abstract

Drying operation is beneficial to the preservation and transportation of microbial ecological agents. In this study, drying kinetics and water distribution variations in solid biomass medium during hot air drying (HAD) and vacuum freeze drying (VFD) were systematically investigated. Meanwhile, the effects of different drying strategies on the sporulation of and the titer of microbial ecological agents were compared. The results showed that both HAD and VFD induced rapid water removal from the solid biomass medium. VFD retained bound water and maintained the porous structure of the solid medium. Both HAD and VFD induced sporulation. The expression level of sporulation-regulatory genes , and followed the order 80°C-HAD > 60°C-HAD > VFD. The spore number in the medium after 80°C-HAD drying for 6 h was 0.72 × 10/g dry medium, which was 9.1 and 12.5% larger than that of the medium with 60°C-HAD and VFD, respectively. Therefore, 80°C-HAD is an effective drying strategy for promoting sporulation, which improves the titer of microbial ecological agents with .

摘要

干燥操作有利于微生物生态制剂的保存和运输。在本研究中,系统研究了热风干燥(HAD)和真空冷冻干燥(VFD)过程中固体生物质培养基的干燥动力学和水分分布变化。同时,比较了不同干燥策略对微生物生态制剂的孢子形成和效价的影响。结果表明,HAD和VFD均能使固体生物质培养基中的水分快速去除。VFD保留了结合水并维持了固体培养基的多孔结构。HAD和VFD均能诱导孢子形成。孢子形成调控基因、和的表达水平顺序为80°C-HAD > 60°C-HAD > VFD。80°C-HAD干燥6 h后培养基中的孢子数为0.72×10/g干培养基,分别比60°C-HAD和VFD培养基中的孢子数多9.1%和12.5%。因此,80°C-HAD是促进孢子形成的有效干燥策略,可提高含微生物生态制剂的效价。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce64/9551345/904e7cf9342e/fnut-09-1025248-g0001.jpg

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