评估基于 NK 细胞的异种肺肿瘤模型中 KIR3DL3 阻断的抗肿瘤活性的方案。
Protocol for evaluating antitumor activity of KIR3DL3 blockade in an NK cell-based xenogeneic lung tumor model.
机构信息
Department of Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Department of Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Oncology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Urology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
出版信息
STAR Protoc. 2022 Nov 4;3(4):101818. doi: 10.1016/j.xpro.2022.101818. eCollection 2022 Dec 16.
Human killer cell immunoglobulin-like receptor, three Ig domains and long cytoplasmic tail (KIR3DL3) is expressed on natural killer (NK) cells and is a newly identified inhibitory receptor for B7 family member HERV-H LTR-associating protein 2 (HHLA2). Here, we summarize the isolation and expansion of KIR3DL3 human NK cells, and functional characterization of in-house anti-KIR3DL3 monoclonal antibody (mAb). We also describe a human NK cell-based xenogeneic lung tumor model for testing the therapeutic activity of KIR3DL3 blockade . For complete details on the use and execution of this protocol, please refer to Wei et al. (2021).
人类杀伤细胞免疫球蛋白样受体，三个 Ig 结构域和长胞质尾（KIR3DL3）表达于自然杀伤（NK）细胞上，是 B7 家族成员人类逆转录病毒（HERV）-H 长末端重复相关蛋白 2（HHLA2）的新鉴定的抑制性受体。在这里，我们总结了 KIR3DL3 人 NK 细胞的分离和扩增，以及内部抗-KIR3DL3 单克隆抗体（mAb）的功能特征。我们还描述了一种基于人 NK 细胞的异种肺肿瘤模型，用于测试 KIR3DL3 阻断的治疗活性。有关该方案使用和执行的完整详细信息，请参见 Wei 等人（2021 年）。
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