利用 CRISPR-Cas9 编辑和 RNA 干扰研究大豆 14-3-3 基因（）在对白粉病抗性和结瘤方面的作用。

The Role of a Soybean 14-3-3 Gene () on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA Silencing.

机构信息

Ottawa Research and Development Centre, Science and Technology, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada.

United States Department of Agriculture-Agricultural Research Service, Urbana, IL 61801, U.S.A.

出版信息

Mol Plant Microbe Interact. 2023 Mar;36(3):159-164. doi: 10.1094/MPMI-07-22-0157-R. Epub 2023 Feb 6.

DOI:10.1094/MPMI-07-22-0157-R

PMID:36428245

Abstract

The role of a soybean 14-3-3 gene () in defense against white mold and in nodulation was investigated by loss-of-gene-function with CRISPR-Cas9 editing and silencing of RNA interference (RNAi). Particle bombardment was used to introduce the CRISPR expression cassette to target the soybean 14-3-3 gene and an RNAi construct to silence gene transcription. Transmission of the edited 14-3-3 gene and the RNAi construct was confirmed in their respective progeny. The recovered transgenic plants and their progeny were significantly more susceptible to infection and showed a significant reduction in nodulation, thus confirming the role of the 14-3-3 gene () in both nodulation and defense.

摘要

利用 CRISPR-Cas9 编辑和 RNA 干扰 (RNAi) 沉默技术研究了大豆 14-3-3 基因在对白粉病的防御和结瘤中的作用。利用粒子轰击将 CRISPR 表达盒导入大豆 14-3-3 基因的靶标，并通过 RNAi 构建物沉默基因转录。在各自的后代中证实了编辑的 14-3-3 基因和 RNAi 构建物的传递。回收的转基因植物及其后代对感染的敏感性显著增加，结瘤显著减少，从而证实了 14-3-3 基因在结瘤和防御中的作用。