人类 2/3 层锥体神经元树突 NMDA 棘波产生的高突触阈值。
High synaptic threshold for dendritic NMDA spike generation in human layer 2/3 pyramidal neurons.
机构信息
John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia.
Florey Institute of Neuroscience and Mental Health, University of Melbourne, Melbourne, VIC, Australia; Trinity College Institute for Neuroscience, Trinity College Dublin, Dublin, Ireland.
出版信息
Cell Rep. 2022 Dec 13;41(11):111787. doi: 10.1016/j.celrep.2022.111787.
Neurons receive synaptic input primarily onto their dendrites. While we know much about the electrical properties of dendrites in rodents, we have only just started to describe their properties in the human brain. Here, we investigate the capacity of human dendrites to generate NMDA-receptor-dependent spikes (NMDA spikes). Using dendritic glutamate iontophoresis, as well as local dendritic synaptic stimulation, we find that human layer 2/3 pyramidal neurons can generate dendritic NMDA spikes. The capacity to evoke NMDA spikes in human neurons, however, was significantly reduced compared with that in rodents. Simulations in morphologically realistic and simplified models indicated that human neurons have a higher synaptic threshold for NMDA spike generation primarily due to the wider diameter of their dendrites. In summary, we find reduced NMDA spike generation in human compared with rodent layer 2/3 pyramidal neurons and provide evidence that this is due to the wider diameter of human dendrites.
神经元主要在其树突上接收突触输入。虽然我们对啮齿动物树突的电特性了解很多，但我们才刚刚开始描述人类大脑中的树突特性。在这里，我们研究了人类树突产生 NMDA 受体依赖性尖峰（NMDA 尖峰）的能力。使用树突谷氨酸离子电泳以及局部树突突触刺激，我们发现人类 2/3 层锥体神经元可以产生树突 NMDA 尖峰。然而，与啮齿动物相比，人类神经元产生 NMDA 尖峰的能力显著降低。在形态逼真和简化模型中的模拟表明，人类神经元产生 NMDA 尖峰的突触阈值更高，主要是由于其树突直径较宽。总之，我们发现与啮齿动物 2/3 层锥体神经元相比，人类 NMDA 尖峰生成减少，并提供证据表明这是由于人类树突直径较宽所致。
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