diffusible signaling molecules enhance biofilm formation gene expression modulation.

BACKGROUND

Periodontitis is caused by a dysbiotic shift in the dental plaque microbiome. is involved in the colonization of , which plays a key role in dysbiosis, via coaggregation and synergy with this microorganism.

AIM

We investigated the effect of diffusible signaling molecules from ATCC 33277 on TDC 100 to elucidate the synergistic mechanisms involved in dysbiosis.

METHODS

The two species were cocultured separated with an 0.4-µm membrane in tryptic soy broth, and gene expression profiles in coculture with were compared with those in monoculture.

RESULTS

RNA sequencing revealed 139 genes differentially expressed between the coculture and monoculture. The expression of 52 genes was upregulated, including the coaggregation ligand-coding gene. Eighty-seven genes were downregulated. Gene Ontology analysis indicated enrichment for the glycogen synthesis pathway and a decrease in de novo synthesis of purine and pyrimidine.

CONCLUSION

These results indicate that diffusible signaling molecules from induce metabolic changes in , including an increase in polysaccharide synthesis and reduction in de novo synthesis of purine and pyrimidine. The metabolic changes may accelerate biofilm formation by with . Further, the alterations may represent potential therapeutic targets for preventing dysbiosis.