莲房原花青素通过 CREB-BDNF 通路介导的 LTP 改善转基因小鼠认知障碍
Procyanidins Extracted from the Lotus Seedpod Ameliorate Cognitive Impairment through CREB-BDNF Pathway Mediated LTP in Transgenic Mice.
机构信息
Department of Nutrition Hygiene and Toxicology, Academy of Nutrition and Health, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, School of Public Health, Wuhan University of Science and Technology, Wuhan, 430065, China.
Department of Neurology, Xiangyang No.1 People's Hospital, Hubei University of Medicine, Xiangyang, 441100, China.
出版信息
Curr Pharm Biotechnol. 2023;24(12):1560-1567. doi: 10.2174/1389201024666230209142145.
BACKGROUND
Alzheimer's disease (AD) is an age-related neurodegenerative disease and is featured by cognitive impairment. Procyanidins have been shown to have a potential protective effect against neurodegenerative diseases, but the underlying mechanism is not comprehensive enough.
OBJECTIVE
To further investigate the effects of procyanidins from lotus seedpod (LSPC) on cognition in AD.
METHODS
The transgenic mice were administered with LSPC (100 mg/kg body weight) for five months. The Morris water maze test was used to assess learning and memory function, the long-term potentiation (LTP) was measured, and the expressions of Aβ, pCREB/CREB and BDNF were quantified by western blot.
RESULTS
LSPC significantly ameliorated cognitive dysfunction, reduced Aβ deposition and reversed the remarkable reduction of the phosphorylation of CREB and the expression of BDNF, and then enhanced the effect of LTP in mice.
CONCLUSION
These results revealed that LSPC could ameliorate cognitive impairment through the CREB-BDNF pathway that mediates the enhancement of LTP in transgenic mice.
背景
阿尔茨海默病（AD）是一种与年龄相关的神经退行性疾病，其特征是认知障碍。原花青素已被证明对神经退行性疾病具有潜在的保护作用，但作用机制还不够全面。
目的
进一步研究莲子原花青素（LSPC）对 AD 认知的影响。
方法
给予转基因小鼠 LSPC（100mg/kg 体重）五个月。采用 Morris 水迷宫试验评估学习记忆功能，测量长时程增强（LTP），并用 Western blot 定量检测 Aβ、pCREB/CREB 和 BDNF 的表达。
结果
LSPC 显著改善认知功能障碍，减少 Aβ 沉积，并逆转 CREB 磷酸化和 BDNF 表达的显著降低，进而增强 转基因小鼠的 LTP 效应。
结论
这些结果表明，LSPC 可通过 CREB-BDNF 通路改善认知障碍，该通路介导增强 转基因小鼠的 LTP。
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