SARS-CoV-2 阿尔法、德尔塔和奥密克戎变异株感染患者的总 RNA 和亚基因组 RNA 病毒载量。
Total and Subgenomic RNA Viral Load in Patients Infected With SARS-CoV-2 Alpha, Delta, and Omicron Variants.
机构信息
Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.
Department of Biostatistics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.
出版信息
J Infect Dis. 2023 Aug 11;228(3):235-244. doi: 10.1093/infdis/jiad061.
BACKGROUND
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomic and subgenomic RNA levels are frequently used as a correlate of infectiousness. The impact of host factors and SARS-CoV-2 lineage on RNA viral load is unclear.
METHODS
Total nucleocapsid (N) and subgenomic N (sgN) RNA levels were measured by quantitative reverse transcription polymerase chain reaction (RT-qPCR) in specimens from 3204 individuals hospitalized with coronavirus disease 2019 (COVID-19) at 21 hospitals. RT-qPCR cycle threshold (Ct) values were used to estimate RNA viral load. The impact of time of sampling, SARS-CoV-2 variant, age, comorbidities, vaccination, and immune status on N and sgN Ct values were evaluated using multiple linear regression.
RESULTS
Mean Ct values at presentation for N were 24.14 (SD 4.53) for non-variants of concern, 25.15 (SD 4.33) for Alpha, 25.31 (SD 4.50) for Delta, and 26.26 (SD 4.42) for Omicron. N and sgN RNA levels varied with time since symptom onset and infecting variant but not with age, comorbidity, immune status, or vaccination. When normalized to total N RNA, sgN levels were similar across all variants.
CONCLUSIONS
RNA viral loads were similar among hospitalized adults, irrespective of infecting variant and known risk factors for severe COVID-19. Total N and subgenomic RNA N viral loads were highly correlated, suggesting that subgenomic RNA measurements add little information for the purposes of estimating infectivity.
背景
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）基因组和亚基因组 RNA 水平常被用作传染性的相关指标。宿主因素和 SARS-CoV-2 谱系对 RNA 病毒载量的影响尚不清楚。
方法
通过定量逆转录聚合酶链反应（RT-qPCR）测量 21 家医院 3204 名因 2019 年冠状病毒病（COVID-19）住院患者的标本中的总核衣壳（N）和亚基因组 N（sgN）RNA 水平。RT-qPCR 循环阈值（Ct）值用于估计 RNA 病毒载量。使用多元线性回归评估采样时间、SARS-CoV-2 变体、年龄、合并症、疫苗接种和免疫状态对 N 和 sgN Ct 值的影响。
结果
非关注变体的 N 出现时的平均 Ct 值为 24.14（SD 4.53），Alpha 变体为 25.15（SD 4.33），Delta 变体为 25.31（SD 4.50），Omicron 变体为 26.26（SD 4.42）。N 和 sgN RNA 水平随症状出现后时间和感染变体而变化，但与年龄、合并症、免疫状态或疫苗接种无关。当与总 N RNA 归一化时，所有变体的 sgN 水平相似。
结论
住院成人的 RNA 病毒载量相似，无论感染变体和 COVID-19 严重程度的已知危险因素如何。总 N 和亚基因组 RNA N 病毒载量高度相关，表明亚基因组 RNA 测量对估计传染性几乎没有增加信息。
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