Genome-Wide Pathway Exploration of the EPI-7.

Functional cosmetics industries using skin microbiome screening and beneficial materials isolated from key microorganisms are receiving increasing attention. Since EPI-7 was first discovered in human skin, previous studies have confirmed that it can produce a new pyrimidine compound, 1,1'-biuracil, having anti-aging effects on human skin. Therefore, we conducted genomic analyses to judge the use value of EPI-7 and provide up-to-date information. Whole-genome sequencing analysis of EPI-7 was performed to generate new complete genome and annotation information. EPI-7 genome was subjected to comparative genomic analysis with a group of closely-related strains and skin flora strains through bioinformatic analysis. Furthermore, based on annotation information, we explored metabolic pathways for valuable substances that can be used in functional cosmetics. In this study, the whole-genome sequencing (WGS) and annotation results of EPI-7 were improved, and through comparative analysis, it was confirmed that the EPI-7 has more metabolite-related genes than comparison strains. In addition, we annotated the vital genes for biosynthesis of 20 amino acids, orotic acid, riboflavin (B2) and chorismate. In particular, we were able to prospect that orotic acid could accumulate inside EPI-7 under uracil-enriched conditions. Therefore, through a genomics approach, this study aims to provide genetic information for the hidden potential of EPI-7 and the strain development and biotechnology utilization to be conducted in further studies.