脯氨酸预处理和化学编程冰核保护球体在冷冻保存过程中。
Proline-conditioning and chemically-programmed ice nucleation protects spheroids during cryopreservation.
机构信息
Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK.
Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China.
出版信息
Chem Commun (Camb). 2023 Jul 20;59(59):9086-9089. doi: 10.1039/d3cc02252h.
Spheroids mimic 3-D tissue niches better than standard cell cultures. Cryopreserving spheroids, however, remains challenging as conventional cryoprotectants do not mitigate all damage mechanisms. Here chemically-programmed extracellular ice nucleation is used to prevent supercooling, alongside proline pre-conditioning, which are found to synergystically improve post-thaw recovery of spheroids. This validates the need to identify compounds and materials to address both biochemical and biophysical damage pathways beyond standard cryoprotectants.
球状体比标准细胞培养更能模拟 3-D 组织龛。然而，冷冻保存球状体仍然具有挑战性，因为传统的冷冻保护剂并不能减轻所有的损伤机制。在这里，化学编程的细胞外冰核形成被用来防止过冷，同时脯氨酸预处理被发现协同提高球状体的解冻后恢复。这证明了需要确定化合物和材料，以解决标准冷冻保护剂之外的生化和生物物理损伤途径。
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