纳米颗粒与细胞相互作用的高通量单细胞分析
High-Throughput Single-Cell Analysis of Nanoparticle-Cell Interactions.
作者信息
Haddad Majood, Frickenstein Alex, Wilhelm Stefan
机构信息
Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma, 73019, USA.
Stephenson Cancer Center, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, 73104, USA.
出版信息
Trends Analyt Chem. 2023 Sep;166. doi: 10.1016/j.trac.2023.117172. Epub 2023 Jul 10.
Understanding nanoparticle-cell interactions at single-nanoparticle and single-cell resolutions is crucial to improving the design of next-generation nanoparticles for safer, more effective, and more efficient applications in nanomedicine. This review focuses on recent advances in the continuous high-throughput analysis of nanoparticle-cell interactions at the single-cell level. We highlight and discuss the current trends in continual flow high-throughput methods for analyzing single cells, such as advanced flow cytometry techniques and inductively coupled plasma mass spectrometry methods, as well as their intersection in the form of mass cytometry. This review further discusses the challenges and opportunities with current single-cell analysis approaches and provides proposed directions for innovation in the high-throughput analysis of nanoparticle-cell interactions.
在单纳米颗粒和单细胞分辨率水平上理解纳米颗粒与细胞的相互作用，对于改进下一代纳米颗粒的设计至关重要，以便在纳米医学中实现更安全、更有效和更高效的应用。本综述重点关注单细胞水平上纳米颗粒与细胞相互作用的连续高通量分析的最新进展。我们着重介绍并讨论用于分析单细胞的连续流动高通量方法的当前趋势，如先进的流式细胞术技术和电感耦合等离子体质谱法，以及它们以质谱流式细胞术形式的交叉融合。本综述还讨论了当前单细胞分析方法所面临的挑战与机遇，并为纳米颗粒与细胞相互作用的高通量分析中的创新提供了建议方向。
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