miR-200 家族成员在龈沟液中用于慢性牙周炎的诊断潜力：与临床参数的相关性及其治疗意义。
Diagnostic potential of miR-200 family members in gingival crevicular fluid for chronic periodontitis: correlation with clinical parameters and therapeutic implications.
机构信息
HangZhou Dental Hospital, HangZhou, 310000, Zhejiang, China.
出版信息
BMC Oral Health. 2023 Jul 31;23(1):532. doi: 10.1186/s12903-023-03174-w.
OBJECTIVE
The purpose of this study was to evaluate the potential of miR-200 family members in gingival crevicular fluid (GCF) as diagnostic biomarkers for chronic periodontitis (CP), aiming to provide valuable insights for the early detection and management of the disease.
METHODS
GSE89081 dataset profiled miRNAs in GCF derived from 5 healthy and 5 periodontitis was analyzed by GEO2R. Quantitative real-time PCR was used to quantify the expression levels of miR-200 family members (miR-200a-3p, miR-200a-5p, miR-200b-3p, miR-200b-5p, miR-200c-3p, miR-200c-5p, miR-141-3p, miR-141-5p, and miR-429) in the GCF samples from 103 CP patients and 113 healthy controls. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic potential of miR-200 family members in differentiating CP patients from healthy controls.
RESULTS
By analyzing the GSE89081 dataset, miR-200a-5p, miR-200b-5p and miR-200c-5p were significantly upregulated in GCF of the CP patients compared to the healthy control. In this study, miR-200a-3p, miR-200a-5p, miR-200b-3p, miR-200b-5p, miR-200c-3p, miR-200c-5p were significantly increased in GCF of CP patients compared to the healthy control, while miR-141 and miR-429 did not show significant differences. MiR-200a, -200b and 200c had good diagnostic value, and when these miRNAs were combined, they demonstrated excellent diagnostic value for CP with an AUC of 0.997, sensitivity of 99.03%, and specificity of 98.23%. MiR-200a, -200b and 200c in GCF showed significant and positive correlation with plaque index (PI), gingival index (GI), bleeding on probing (BOP), clinical attachment level (CAL), and probing pocket depth (PPD).
CONCLUSION
MiR-200a, -200b and 200c in GCF may serve as potential biomarkers for the early diagnosis of CP, which was correlated with clinical parameters, being therapeutic targets for CP.
目的
本研究旨在评估 miR-200 家族成员在龈沟液（GCF）中的潜在作用，作为慢性牙周炎（CP）的诊断生物标志物，为疾病的早期检测和管理提供有价值的见解。
方法
通过 GEO2R 分析 GSE89081 数据集，对 5 名健康和 5 名牙周炎患者的 GCF 中 miRNA 进行分析。使用定量实时 PCR 定量分析 miR-200 家族成员（miR-200a-3p、miR-200a-5p、miR-200b-3p、miR-200b-5p、miR-200c-3p、miR-200c-5p、miR-141-3p、miR-141-5p 和 miR-429）在 103 例 CP 患者和 113 例健康对照者的 GCF 样本中的表达水平。使用受试者工作特征（ROC）曲线分析评估 miR-200 家族成员在区分 CP 患者和健康对照者方面的诊断潜力。
结果
通过分析 GSE89081 数据集，发现 CP 患者的 GCF 中 miR-200a-5p、miR-200b-5p 和 miR-200c-5p 明显上调。在这项研究中，与健康对照组相比，CP 患者的 GCF 中 miR-200a-3p、miR-200a-5p、miR-200b-3p、miR-200b-5p、miR-200c-3p 和 miR-200c-5p 明显增加，而 miR-141 和 miR-429 没有显示出明显差异。miR-200a、-200b 和 200c 具有良好的诊断价值，当这些 miRNA 结合使用时，它们对 CP 的诊断价值非常出色，AUC 为 0.997，灵敏度为 99.03%，特异性为 98.23%。GCF 中的 miR-200a、-200b 和 200c 与菌斑指数（PI）、牙龈指数（GI）、探诊出血（BOP）、临床附着水平（CAL）和探诊袋深度（PPD）呈显著正相关。
结论
GCF 中的 miR-200a、-200b 和 200c 可能作为 CP 早期诊断的潜在生物标志物，与临床参数相关，是 CP 的治疗靶点。
Zotero 插件