一种脂氧合酶样酶对于毒力和与宿主免疫反应相关的定位改变是必需的。

A lipoxygenase-like enzyme is necessary for virulence and changes localization associated with the host immune response.

机构信息

Department of Medical Microbiology and Immunology, University of Wisconsin-Madison , Madison, Wisconsin, USA.

出版信息

mBio. 2023 Oct 31;14(5):e0127923. doi: 10.1128/mbio.01279-23. Epub 2023 Aug 30.

DOI:10.1128/mbio.01279-23

PMID:37646522

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10653942/

Abstract

Lipoxygenases (LOXs) are enzymes that catalyze the deoxygenation of polyunsaturated fatty acids such as linoleic and arachidonic acid. These modifications create signaling molecules that are best characterized for modulating the immune response. Deletion of the first lipoxygenase-like enzyme characterized for (TgLOXL1) generated a less virulent strain, and infected mice showed a decreased immune response. This virulence defect was dependent on the mouse cytokine interferon gamma IFNγ. TgLOXL1 changes location from inside the parasite in tissue culture conditions to vesicular structures within the host immune cells during mouse infection. These results suggest that TgLOXL1 plays a role in the modification of the host immune response in mice.

摘要

脂氧合酶（LOXs）是一种能够催化多不饱和脂肪酸（如亚油酸和花生四烯酸）脱氧的酶。这些修饰产物可以生成信号分子，这些信号分子在调节免疫反应方面表现得尤为突出。首次鉴定的脂氧合酶样酶 1（TgLOXL1）的缺失会产生毒力较弱的菌株，感染的小鼠表现出免疫反应降低。这种毒力缺陷依赖于老鼠细胞因子干扰素 γ IFNγ。在组织培养条件下，TgLOXL1 的位置从寄生虫内部转移到感染小鼠的宿主免疫细胞的囊泡结构中。这些结果表明，TgLOXL1 在调节小鼠的宿主免疫反应中发挥作用。