将 AbsoluteIDQ p180 试剂盒适应于人房水代谢产物分析。

Adaptation of the AbsoluteIDQ p180 kit to the analysis of metabolites in the human aqueous humor.

机构信息

Metabolomics Laboratory, Clinical Research Centre, Medical University of Bialystok, 15-276 Bialystok, Poland.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2023 Sep 1;1229:123880. doi: 10.1016/j.jchromb.2023.123880. Epub 2023 Sep 8.

DOI:10.1016/j.jchromb.2023.123880

PMID:37690387

Abstract

The aim of this study was to use the commercial kit AbsoluteIDQ p180 (Biocrates) for the quantification of metabolites in aqueous humor (AH), as well as to determine the optimal volume of AH that is necessary to obtain reliable and reproducible results. Different volumes of AH (10 µl, 20 µl, and 30 µl) were tested. Of the 188 metabolites measurable with the Biocrates kit, 69 were detected in AH. Depending on the volume used, 41, 51, and 63 metabolites were measured using 10 µl, 20 µl, and 30 µl of AH, respectively. The repeatability of the measurements improved with increasing AH volume. Considering only those metabolites that were obtained with a CV < 15%, 34 metabolites at 10 µl, 41 at 20 µl, and 44 at 30 µl AH were received. On this basis, it can be concluded that the tested method can be successfully applied to analyze metabolites in the human AH. To achieve the most comprehensive detection range and highest repeatability of measurements, it is recommended to use 30 µl AH.

摘要

本研究旨在使用商业试剂盒 AbsoluteIDQ p180（Biocrates）定量分析房水中的代谢物，并确定获得可靠和可重复结果所需的最佳房水体积。测试了不同体积的房水（10 μl、20 μl 和 30 μl）。在 Biocrates 试剂盒可测量的 188 种代谢物中，有 69 种在房水中被检测到。根据使用的体积，分别使用 10 μl、20 μl 和 30 μl 的房水测量了 41、51 和 63 种代谢物。随着房水体积的增加，测量的重复性提高。仅考虑那些变异系数（CV）<15%的代谢物，在 10 μl 的房水中获得了 34 种代谢物，在 20 μl 的房水中获得了 41 种代谢物，在 30 μl 的房水中获得了 44 种代谢物。在此基础上，可以得出结论，所测试的方法可以成功地应用于分析人眼房水中的代谢物。为了实现最全面的检测范围和最高的测量重复性，建议使用 30 μl 的房水。