Evaluation of the Inhibitory Effect of Leaves Methanolic Extract against Growth of Several Species and and the Growth of .

The current study evaluated the inhibitory effect of leaves methanolic extract (MOL) against the growth of (), , , and (), as well as growth of in mice. Active principles of MOL extract were determined using liquid chromatography mass spectrometry (LC-MS). MOL's anti-piroplasm efficacy was assessed both and using the SYBR Green I fluorescence assay. Every 96 hours, the hematological parameters, including red blood cell count (RBCs; 10/UL), hemoglobin content (HGB; g/dl), and hematocrit percent (HCT; %), in the treated mice were monitored using a Celltac MEK6450 automated hematological analyzer. LC-MS of MOL revealed that the most abundant polyphenolic catechism found in the MOL extract was isoquercetin and rutin. MOL inhibited , and growth in a dose-dependent way, with IC values of 45.29 ± 6.14, 19.16 ± 0.45, 137.49 ± 16.07, and 9.29 ± 0.014 g/ml, respectively. MOL's antibabesial activity was enhanced when administrated simultaneously with either diminazene aceturate (DA) or MMV665875 compound from malaria box. In mice infected by , a combination of MOL and a low dose of DA (12.5 mg·kg) resulted in a significant ( < 0.05) reduction in growth. These findings suggest that MOL is an effective herbal anti-piroplasm therapy, especially when combined with a low dosage of either DA or MMV665875.