关于冷冻电镜数据存档与验证的社区建议：wwPDB/EMDB冷冻电镜数据管理、提交与验证研讨会成果
Community recommendations on cryoEM data archiving and validation: Outcomes of a wwPDB/EMDB workshop on cryoEM data management, deposition and validation.
作者信息
Kleywegt Gerard J, Adams Paul D, Butcher Sarah J, Lawson Catherine L, Rohou Alexis, Rosenthal Peter B, Subramaniam Sriram, Topf Maya, Abbott Sanja, Baldwin Philip R, Berrisford John M, Bricogne Gérard, Choudhary Preeti, Croll Tristan I, Danev Radostin, Ganesan Sai J, Grant Timothy, Gutmanas Aleksandras, Henderson Richard, Heymann J Bernard, Huiskonen Juha T, Istrate Andrei, Kato Takayuki, Lander Gabriel C, Lok Shee-Mei, Ludtke Steven J, Murshudov Garib N, Pye Ryan, Pintilie Grigore D, Richardson Jane S, Sachse Carsten, Salih Osman, Scheres Sjors H W, Schroeder Gunnar F, Sorzano Carlos Oscar S, Stagg Scott M, Wang Zhe, Warshamanage Rangana, Westbrook John D, Winn Martyn D, Young Jasmine Y, Burley Stephen K, Hoch Jeffrey C, Kurisu Genji, Morris Kyle, Patwardhan Ardan, Velankar Sameer
机构信息
EMBL-EBI, Cambridge, UK.
Lawrence Berkeley Laboratory, Berkeley, CA, USA and University of California, Berkeley, CA, USA.
出版信息
ArXiv. 2024 Feb 2:arXiv:2311.17640v3.
In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data requirements for deposition and validation of cryoEM structures, with a focus on single-particle analysis. The meeting was attended by 47 experts in data processing, model building and refinement, validation, and archiving of such structures. This report describes the workshop's motivation and history, the topics discussed, and consensus recommendations resulting from the workshop. Some challenges for future methods-development efforts in this area are also highlighted, as is the implementation to date of some of the recommendations.
2020年1月，欧洲生物信息学研究所（英国欣克斯顿）举办了一场研讨会，讨论冷冻电镜结构的存档与验证的数据要求，重点是单颗粒分析。47位数据处理、模型构建与优化、验证以及此类结构存档方面的专家参加了此次会议。本报告介绍了研讨会的动机和背景、讨论的主题以及研讨会达成的共识性建议。还强调了该领域未来方法开发工作面临的一些挑战，以及部分建议到目前的实施情况。
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