一个非神经 miRNA 簇通过抑制两个神经靶标来介导听力。

A nonneural miRNA cluster mediates hearing via repression of two neural targets.

机构信息

Developmental Biology Program, Sloan Kettering Institute, New York, New York 10065, USA.

Department of Biology, Colby College, Waterville, Maine 04901, USA.

出版信息

Genes Dev. 2023 Dec 26;37(21-24):1041-1051. doi: 10.1101/gad.351052.123.

DOI:10.1101/gad.351052.123

PMID:38110249

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10760640/

Abstract

We show here that are absolutely essential for development and function of Johnston's organ (JO), the primary proprioceptive and auditory organ in Their deletion results in highly aberrant cell fate determination, including loss of scolopale cells and ectopic neurons, and mutants are electrophysiologically deaf. In vivo activity sensors and mosaic analyses indicate that these seed-related miRNAs function autonomously to suppress neural fate in nonneuronal cells. Finally, genetic interactions pinpoint two neural targets ( and ) that underlie miRNA mutant JO phenotypes. This work uncovers how critical post-transcriptional regulation of specific miRNA targets governs cell specification and function of the auditory system.

摘要

我们在这里表明，在 Johnston 器官（JO）的发育和功能中是绝对必需的，JO 是的主要本体感受和听觉器官。它们的缺失导致高度异常的细胞命运决定，包括栉板细胞和异位神经元的丧失，并且突变体在电生理学上是聋的。体内活性传感器和嵌合体分析表明，这些与种子相关的 miRNA 独立地发挥作用，以抑制非神经元细胞中的神经命运。最后，遗传相互作用确定了两个神经靶标（和），它们是 miRNA 突变体 JO 表型的基础。这项工作揭示了特定 miRNA 靶标的关键转录后调控如何控制听觉系统的细胞特化和功能。